AIL and HDG proteins act antagonistically to control cell proliferation
AIL and HDG proteins act antagonistically to control cell proliferation
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DOI：
10.1242/dev.117168
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发表时间：
2015-02-01
期刊：
影响因子：
4.6
通讯作者：
Boutilier, Kim
中科院分区：
文献类型：
--
作者：
Horstman, Anneke;Fukuoka, Hiroyuki;Boutilier, Kim
AINTEGUMENTA-like(AINTEGUMENTA-like)转录因子是细胞增殖和分生组织特性的关键调节因子。虽然All的功能已经被很好地描述，但这个途径的直接信号成分在很大程度上是未知的。我们发现婴儿潮(BBM)和其他AIL蛋白与L1表达的同源结构域无毛(HDG)转录因子家族的多个成员物理上相互作用，包括HDG1、HDG11和HDG12。HDG1、HDG11和HDG12的过表达由于根和茎分生组织停滞而限制生长，这与分生组织发育和细胞增殖途径相关的基因表达减少有关，而多个HDG基因的下调促进了细胞的过度增殖。这些结果表明，HDG蛋白在促进细胞分化方面发挥了作用。我们还揭示了一个转录网络，其中BBM和HDG1调节几个共同的靶基因，BBM/AIL和HDG相互调节表达。综上所述，这些结果表明AIL和HDG蛋白具有相反的作用，AIL促进细胞增殖，HDG刺激细胞分化，这些功能是在蛋白质-蛋白质相互作用和转录水平上调节的。
AINTEGUMENTA-LIKE (AIL) transcription factors are key regulators of cell proliferation and meristem identity. Although AIL functions have been well described, the direct signalling components of this pathway are largely unknown. We show that BABY BOOM (BBM) and other AIL proteins physically interact with multiple members of the L1-expressed HOMEODOMAIN GLABROUS (HDG) transcription factor family, including HDG1, HDG11 and HDG12. Overexpression of HDG1, HDG11 and HDG12 restricts growth due to root and shoot meristem arrest, which is associated with reduced expression of genes involved in meristem development and cell proliferation pathways, whereas downregulation of multiple HDG genes promotes cell overproliferation. These results suggest a role for HDG proteins in promoting cell differentiation. We also reveal a transcriptional network in which BBM and HDG1 regulate several common target genes, and where BBM/AIL and HDG regulate the expression of each other. Taken together, these results suggest opposite roles for AIL and HDG proteins, with AILs promoting cell proliferation and HDGs stimulating cell differentiation, and that these functions are mediated at both the protein-protein interaction and transcriptional level.