Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus
Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus
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山东粘液虫 (Myxobolus shantungensis Hu) 的重新描述和分子分析，1965（粘虫纲：粘孢子纲）感染鲤鱼 Cyprinus carpio haematopterus
DOI：
10.1007/s00436-011-2450-0
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发表时间：
2011-12-01
影响因子：
2
通讯作者：
Zeng, Lingbing
中科院分区：
文献类型：
--
作者：
Liu, Yang;Gu, Zemao;Zeng, Lingbing
粘虫（Myxobolus shantungensis Hu, 1965）侵染鲤鱼（Cyprinus carpio haematopterus, Temminck and Schlegel）的鳃弓，对宿主鱼造成严重的病理影响。不充分的原始描述和缺乏分子数据使准确的早期诊断具有挑战性。为了补充原始描述，本文采用形态学和分子生物学方法对山通菌进行了重新描述。山桐孢子成熟孢子正面呈椭圆形或苹果状，侧面呈柠檬状，平均为8.2±0.3 μm （8.0 ~ 9.0 μm） × 10.1±0.5 μm （9.2 ~ 11.1 μm） × 6.9±0.3 μm （6.0 ~ 7.4 μm）。有的孢子在孢子后部有3 ~ 4个“V”形瓣边纹。两个极性相等的蒴果呈椭圆形，尺寸为4.3±0.3 μm （3.8 ~ 5.0 μm） × 3.2±0.2 μm (2.8 ~ 3.5 μm)，位于孢子前端。极丝盘绕六到七圈。扫描电镜观察发现，山通孢子成熟孢子表面普遍有凹坑，有许多不规则的脊状突起。在分子水平上，利用小亚基核糖体RNA基因序列对山东结核分枝杆菌进行了鉴定。BLAST检索显示该序列与GenBank中任何可用序列都不匹配。
Myxobolus shantungensis Hu, 1965 infects gill arches of common carp Cyprinus carpio haematopterus (Temminck and Schlegel), causing serious pathological effects on host fish. An inadequate original description and absence of molecular data make accurate early diagnosis challenging. To augment the original description, M. shantungensis is redescribed here using morphological and molecular biological methods. Mature spores of M. shantungensis were ellipsoidal or apple shaped in frontal view and lemon shaped in lateral view, averaging 8.2 ± 0.3 μm (8.0-9.0 μm) × 10.1 ± 0.5 μm (9.2-11.1 μm) × 6.9 ± 0.3 μm (6.0-7.4 μm). Some spores had three to four "V"-shaped valve edge markings on the posterior of the spore. The two equal polar capsules were oval, measuring 4.3 ± 0.3 μm (3.8-5.0 μm) × 3.2 ± 0.2 μm (2.8-3.5 μm), situated at the anterior extremity of the spore. Polar filaments coiled with six to seven turns. Scanning electron microscopy revealed that the surface of mature spores of M. shantungensis was generally pitted with a number of irregular ridges in shape. M. shantungensis is also characterized on the molecular level using the sequence of the small subunit ribosomal RNA gene. A BLAST search revealed that this sequence did not match any available sequences in GenBank.