Association of an S1 nuclease-sensitive structure with short direct repeats 5′ of Drosophila heat shock genes
Association of an S1 nuclease-sensitive structure with short direct repeats 5′ of Drosophila heat shock genes
复制标题
S1 核酸酶敏感结构与果蝇热休克基因 5 短直接重复序列的关联
作者：
H. A. Mace;H. Pelham;A. Travers
DNA分子具有不同的三级结构的可能性被认为是真核生物1，2转录调控的可能决定因素。这些形式包括十字形3，4在同一分子2，5中从B到Z DNA的转变，以及在交替的共聚物中DNA的短的不成对的延伸6。所有这些结构都包含对S1核酸酶4-6优先敏感的区域。我们在这里报告了在某些果蝇热休克基因的5‘侧翼区域发现的与DNA短直接重复相关的额外一类S1核酸酶敏感部位。
The potential for a DNA molecule to assume different tertiary structures has been suggested as a possible determinant of transcriptional regulation in eukaryotes1,2. Such forms include cruciforms3,4 transitions from B to Z DNA in the same molecule2,5 and short unpaired stretches of DNA in an alternating copolymer6. All such structures contain regions which are preferentially sensitive to S1 nuclease4–6. We report here an additional class of S1 nuclease sensitive sites associated with short direct repeats of DNA found in the 5′ flanking regions of certain Drosophila melanogaster heat shock genes.