Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds
Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds
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DOI：
10.1021/pr100456d
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发表时间：
2010-09-01
影响因子：
4.4
通讯作者：
Fox, Jay W.
中科院分区：
文献类型：
--
作者：
Eming, Sabine A.;Koch, Manuel;Fox, Jay W.
在西方国家，与血管疾病、糖尿病或衰老相关的慢性伤口是发病的主要原因，并且代表未解决的临床问题。因此，开发创新策略以促进组织修复是一项重要任务，需要对潜在的分子病理生理学进行更彻底的分析。我们建议，控制组织修复或其失败的复杂的生物事件的理解，在很大程度上受益于广泛的分析方法提供了新的蛋白质组学方法。在这里，我们提出了第一个比较蛋白质组分析伤口渗出液获得正常愈合或不愈合（静脉性腿部溃疡）的人皮肤伤口。共鉴定出149种蛋白质，具有很高的置信度。少数蛋白质仅存在于愈合伤口（23种蛋白质）或未愈合伤口（26种蛋白质）的渗出液中。特别令人感兴趣的是两种不同愈合表型之间特异性蛋白质的差异分布。然而，在从愈合伤口获得的渗出液中，组织形成的介质特征大量存在，而在从非愈合伤口获得的渗出液中，鉴定出许多持续性炎症和组织破坏性反应的介质特征。此外，该研究还揭示了关于鉴定在皮肤修复中具有未知功能的新蛋白质的有趣结果。因此，该分析代表了寻找潜在生物标志物的重要基础，从而更好地理解和监测慢性伤口中的疾病进展。
Chronic wounds associated with vascular disease, diabetes mellitus, or aging are leading causes of morbidity in western countries and represent an unresolved clinical problem. The development of innovative strategies to promote tissue repair is therefore an important task that requires a more thorough analysis of the underlying molecular pathophysiology. We propose that the understanding of the complex biological events that control tissue repair or its failure largely benefits from a broad analytical approach as provided by novel proteomic methodologies. Here we present the first comparative proteome analysis of wound exudates obtained from normal healing or nonhealing (venous leg ulcer) human skin wounds. A total of 149 proteins were identified with high confidence. A minority of proteins was exclusively present in exudate of the healing wound (23 proteins) or the nonhealing wound (26 proteins). Of particular interest was the differential distribution of specific proteins among the two different healing phenotypes. Whereas in the exudate obtained from the healing wound mediators characteristic for tissue formation were abundantly present, in the exudate obtained from the nonhealing wound numerous mediators characteristic for a persistent inflammatory and tissue destructive response were identified. Furthermore, the study also revealed interesting results regarding the identification of new proteins with yet unknown functions in skin repair. This analysis therefore represents an important basis for the search for potential biomarkers, which give rise to a better understanding and monitoring of disease progression in chronic wounds.