Atopic dermatitis-like pruritic skin inflammation caused by feeding a special diet to HR-1 hairless mice
Atopic dermatitis-like pruritic skin inflammation caused by feeding a special diet to HR-1 hairless mice
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DOI：
10.1111/j.0906-6705.2005.00313.x
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发表时间：
2005-06-01
影响因子：
3.6
通讯作者：
Kohno, S
中科院分区：
文献类型：
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作者：
Fujii, M;Tomozawa, J;Kohno, S
皮肤干燥/屏障功能障碍被认为是特应性皮炎（AD）的特征之一。HR-1型无毛小鼠饲喂特殊饲料HR-AD后，皮肤干红。我们通过显示瘙痒相关的抓挠行为和分析免疫变化来检验hr -AD喂养的小鼠是否可以作为AD的模型。HR-1小鼠从4周龄开始饲喂HR-AD。饲喂hr - ad的小鼠在第14天或第28天表现出严重的皮肤干燥症状，并伴有真皮含水量减少、经皮失水增加和抓挠持续时间延长。这些症状逐渐加重，直到第56天。从第28天开始，观察到皮肤表皮明显增生，CD4（+）细胞轻微升高。相反，从第41天开始，循环T细胞和血清免疫球蛋白E增加。其他皮肤浸润性炎症细胞，如嗜酸性粒细胞和肥大细胞，在第56天增加，但在第28天没有增加。虽然每日口服地塞米松治疗可以减少这些细胞数量的增加，但它对皮肤干燥症状或长时间的抓挠发作没有影响。相比之下，饲喂含有10%正常饲料的HR-AD可抑制皮肤干燥的发生。hr - ad喂养小鼠皮肤屏障功能障碍与ad样瘙痒的发生密切相关。观察到的免疫参数的变化可能是皮肤屏障功能障碍的结果。我们的研究结果表明，hr -AD喂养小鼠可以作为AD的干性皮肤实验模型。
Dry skin/barrier dysfunction is considered to be one of the characteristic features of atopic dermatitis (AD). When HR-1 hairless mice are fed a special diet, HR-AD, dry red skin is induced. We examined whether HR-AD-fed mouse could be used as a model for AD by showing itch-associated scratching behaviour and by analysing the immunological change. HR-1 mice were fed HR-AD from 4 weeks old. HR-AD-fed mice showed severe dry skin symptoms accompanied by a decrease in dermal water content and an increase in transepidermal water loss and prolonged scratching bout duration on day 14 or 28. These symptoms became gradually worse until day 56. Marked epidermal hyperplasia and slight increase in CD4(+) cells in the skin were observed from day 28. In contrast, increases in circulating T cells and serum immunoglobulin E were seen from day 41. Other skin-infiltrating inflammatory cells, such as eosinophils and mast cells, were increased on day 56 but not on day 28. Though daily oral treatment with dexamethasone reduced the increased numbers of these cells, it did not affect the dry skin symptoms or the prolonged scratching episodes. In contrast, the development of dry skin was inhibited by feeding with 10% normal diet-containing HR-AD. The skin barrier dysfunction in HR-AD-fed mice is closely associated with the development of AD-like pruritus. Changes in the immunological parameters observed may be the consequence of skin barrier dysfunction. Our findings suggest that HR-AD-fed mouse could be used as a dry skin-based experimental model for AD.