Exosomes in ovarian cancer ascites promote epithelial-mesenchymal transition of ovarian cancer cells by delivery of miR-6780b-5p.
Exosomes in ovarian cancer ascites promote epithelial-mesenchymal transition of ovarian cancer cells by delivery of miR-6780b-5p.
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卵巢癌腹水中的外泌体通过传递 miR-6780b-5p 促进卵巢癌细胞的上皮间质转化
DOI：
10.1038/s41419-021-03490-5
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发表时间：
2021-02-24
影响因子：
9
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Cai J;Gong L;Li G;Guo J;Yi X;Wang Z
卵巢癌的预后差主要是由于转移，而卵巢癌转移的具体机制尚不清楚。腹水来源的外泌体（ADE）在卵巢癌的进展中起重要作用，但其机制尚不清楚。在这里，我们发现ADEs不仅在体外而且在体内促进卵巢癌转移。这种促进功能是基于卵巢癌细胞的上皮间质转化（EMT）。RNA测序微阵列数据的生物信息学分析表明，miR-6780 b-5 p可能是ADE中促进癌症转移的关键微小RNA（miRNA）。此外，外泌体miR-6780 b-5 p的表达与卵巢癌患者的肿瘤转移相关。miR-6780 b-5 p过表达促进卵巢癌细胞EMT，而miR-6780 b-5 p下调抑制卵巢癌细胞EMT。这些结果表明，ADEs将miR-6780 b-5 p转移到卵巢癌细胞，促进EMT并最终促进卵巢癌转移。
The poor prognosis of ovarian cancer is mainly due to metastasis, and the specific mechanism underlying ovarian cancer metastasis is not clear. Ascites-derived exosomes (ADEs) play an important role in the progression of ovarian cancer, but the mechanism is unknown. Here, we found that ADEs promoted ovarian cancer metastasis not only in vitro but also in vivo. This promotive function was based on epithelial–mesenchymal transition (EMT) of ovarian cancer cells. Bioinformatics analysis of RNA sequencing microarray data indicated that miR-6780b-5p may be the key microRNA (miRNA) in ADEs that facilitates cancer metastasis. Moreover, the expression of exosomal miR-6780b-5p correlated with tumor metastasis in ovarian cancer patients. miR-6780b-5p overexpression promoted and miR-6780b-5p downregulation suppressed EMT of ovarian cancer cells. These results suggest that ADEs transfer miR-6780b-5p to ovarian cancer cells, promoting EMT and finally facilitating ovarian cancer metastasis.
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影响因子：
8
作者：
通讯作者：
--
影响因子：
5.4
作者：
Han, Qing;Huang, Bangxing;Wang, Zehua
通讯作者：
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DOI：
10.1038/nrc4019
发表时间：
2015-11
期刊：
Nature reviews. Cancer
影响因子：
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作者：
Bowtell DD;Böhm S;Ahmed AA;Aspuria PJ;Bast RC Jr;Beral V;Berek JS;Birrer MJ;Blagden S;Bookman MA;Brenton JD;Chiappinelli KB;Martins FC;Coukos G;Drapkin R;Edmondson R;Fotopoulou C;Gabra H;Galon J;Gourley C;Heong V;Huntsman DG;Iwanicki M;Karlan BY;Kaye A;Lengyel E;Levine DA;Lu KH;McNeish IA;Menon U;Narod SA;Nelson BH;Nephew KP;Pharoah P;Powell DJ Jr;Ramos P;Romero IL;Scott CL;Sood AK;Stronach EA;Balkwill FR
通讯作者：
Balkwill FR
DOI：
10.1016/s0140-6736(17)33326-3
发表时间：
2018-03-17
期刊：
Lancet (London, England)
影响因子：
--
作者：
Allemani C;Matsuda T;Di Carlo V;Harewood R;Matz M;Nikšić M;Bonaventure A;Valkov M;Johnson CJ;Estève J;Ogunbiyi OJ;Azevedo E Silva G;Chen WQ;Eser S;Engholm G;Stiller CA;Monnereau A;Woods RR;Visser O;Lim GH;Aitken J;Weir HK;Coleman MP;CONCORD Working Group
通讯作者：
CONCORD Working Group
影响因子：
9.7
作者：
Katakowski, Mark;Buller, Ben;Zheng, Xuguang;Lu, Yong;Rogers, Thomas;Osobamiro, Oyinkansola;Shu, Wayne;Jiang, Feng;Chopp, Michael
通讯作者：
Chopp, Michael