MECHANICAL-STRESS STIMULATES AORTIC ENDOTHELIAL-CELLS TO PROLIFERATE
MECHANICAL-STRESS STIMULATES AORTIC ENDOTHELIAL-CELLS TO PROLIFERATE
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DOI：
10.1067/mva.1987.avs0060252
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发表时间：
1987-09-01
影响因子：
4.3
通讯作者：
JOHNSON, G
中科院分区：
文献类型：
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作者：
SUMPIO, BE;BANES, AJ;JOHNSON, G
研究了周期性拉伸变形和松弛对培养的牛主动脉内皮细胞的影响。将第3代至第9代的内皮细胞接种在柔性底板中，并使其贴壁24小时。将实验组中的内皮细胞（每个时间点n = 6个威尔斯孔）置于真空操作的应力提供装置中，该应力提供装置在培养板底部最大向下偏转时施加平均10%的伸长。从培养的第1天到第7天，使拉伸的内皮细胞经历10秒伸长和10秒松弛的重复循环。对照组中的内皮细胞（每个时间点n = 6个威尔斯孔）经受与实验组相似的孵育条件，但没有张力变形。在收获前24小时向细胞中加入氚化胸苷。在第0、1、3、5和7天，对细胞进行计数并分析三氯乙酸可沉淀的氚化胸苷掺入。结果表明，3个周期/分钟的机械拉伸刺激脱氧核糖核酸合成和内皮细胞分裂。我们的结论是，周期性拉伸变形可能会刺激内皮细胞增殖。体内自然发生的周期性机械变形，如心脏对主动脉组织的重复拉伸和松弛，可能会引起内皮细胞的特定合成和分裂模式。（J VASCSurg1987;6：252-6.）
The effects of applied cyclic tensional deformation and relaxation on cultured bovine aortic endothelial cells were examined. Endothelial cells from passages 3 to 9 were seeded in flexible-bottomed plates and allowed to attach for 24 hours. Endothelial cells in the experimental group (n = 6 wells per time point) were placed in a vacuum-operated stress-providing instrument that exerted an average elongation of 10% at maximum downward deflection of the culture plate bottom. The stretched endothelial cells were subjected to repeating cycles of 10 seconds elongation and 10 seconds relaxation from days 1 through 7 in culture. Endothelial cells in the control group (n = 6 wells per time point) were subjected to similar incubation conditions as the experimental group but without tensional deformation. Tritiated thymidine was added to cells 24 hours before harvesting. On days 0, 1, 3, 5, and 7 cells were counted and analyzed for trichloroacetic acid-precipitable tritiated thymidine incorporation. The results showed that 3 cycles/min mechanical stretching stimulated deoxyribonucleic acid synthesis and endothelial cell division. We conclude that cyclic tensional deformation may stimulate endothelial cell proliferation. It is possible that naturally occurring cyclic mechanical deformation in vivo, such as the repetitive stretching and relaxation of aortic tissue by the heart, may invoke a particular pattern of synthesis and division in endothelial cells. (J VASCSURG1987;6:252-6.)