Pathfinding and error correction by retinal axons:: The role of astray/robo2
Pathfinding and error correction by retinal axons:: The role of astray/robo2
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DOI：
10.1016/s0896-6273(01)00579-7
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发表时间：
2002-01-17
期刊：
影响因子：
16.2
通讯作者：
Chien, CB
中科院分区：
文献类型：
--
作者：
Hutson, LD;Chien, CB
为了解决高度定型的视网膜顶盖通路如何在斑马鱼中发展，我们使用固定组织和延时成像来分析野生型和突变型视网膜生长锥的形态和行为。野生型生长锥的复杂性增加，并在中线处暂停。有趣的是，他们偶尔会做出同侧投影和其他寻路错误，这些错误最终都会得到纠正。在missile/robo 2突变体中，生长锥比野生型更大，更复杂。偏离的轴突产生在野生型中看不到的中线错误，以及中线之前和之后的错误。错误很少被纠正。假定的Robo配体Slit 2和Slit 3在通路附近以与其通过Robo 2介导寻路一致的模式表达。因此，Robo 2并不控制视网膜轴突的中线交叉，而是通过防止和纠正寻路错误来塑造它们的路径。
To address how the highly stereotyped retinotectal pathway develops in zebrafish, we used fixed-tissue and time-lapse imaging to analyze morphology and behavior of wild-type and mutant retinal growth cones. Wild-type growth cones increase in complexity and pause at the midline. Intriguingly, they make occasional ipsilateral projections and other path-finding errors, which are always eventually corrected. In the astray/robo2 mutant, growth cones are larger and more complex than wild-type. astray axons make midline errors not seen in wild-type, as well as errors both before and after the midline. astray errors are rarely corrected. The presumed Robo ligands Slit2 and Slit3 are expressed near the pathway in patterns consistent with their mediating pathfinding through Robo2. Thus, Robo2 does not control midline crossing of retinal axons, but rather shapes their pathway, by both preventing and correcting pathfinding errors.