Rapid generation of protein aerosols and nanoparticles via SAW atomisation
Rapid generation of protein aerosols and nanoparticles via SAW atomisation
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DOI：
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发表时间：
2008
期刊：
影响因子：
3.5
通讯作者：
M. Alvarez;J. Friend;L. Yeo
中科院分区：
文献类型：
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作者：
M. Alvarez;J. Friend;L. Yeo
我们描述了表面声波（SAW）雾化器的制造，并显示其产生单分散气溶胶和颗粒的药物输送应用的能力。特别是，我们证明了胰岛素液体气雾剂的肺部输送和固体蛋白质纳米粒子的经皮和胃肠道输送途径，使用20 MHz的SAW器件的生成。获得了约3 μ m的胰岛素液滴，与肺泡区域吸收最大化的最佳范围相匹配。提供了一种新的方法来解释这些雾化液滴直径返回到基本的物理分析，并考虑粘性毛细管和惯性毛细管力的平衡，而不是采用修改的开尔文方程的参数强迫的假设下，已扩展到这些频率在过去的调查。此外，我们考虑可能的机制，通过它的液滴喷射发生的帮助下，高速流动可视化。最后，我们表明，纳米级的蛋白质颗粒（50-100 nm的直径），通过初始气溶胶的蒸发过程中，其最终大小可以控制仅仅通过修改初始蛋白质浓度。这些结果说明了使用SAW作为快速产生具有受控和窄尺寸分布的颗粒和液滴的新方法的可行性
We describe the fabrication of a surface acoustic wave (SAW) atomizer and show its ability to generate monodisperse aerosols and particles for drug delivery applications. In particular, we demonstrate the generation of insulin liquid aerosols for pulmonary delivery and solid protein nanoparticles for transdermal and gastrointestinal delivery routes using 20 MHz SAW devices. Insulin droplets around 3 mu m were obtained, matching the optimum range for maximizing absorption in the alveolar region. A new approach is provided to explain these atomized droplet diameters by returning to fundamental physical analysis and considering viscous-capillary and inertial-capillary force balance rather than employing modifications to the Kelvin equation under the assumption of parametric forcing that has been extended to these frequencies in past investigations. In addition, we consider possible mechanisms by which the droplet ejections take place with the aid of high-speed flow visualization. Finally, we show that nanoscale protein particles (50-100 nm in diameter) were obtained through an evaporative process of the initial aerosol, the final size of which could be controlled merely by modifying the initial protein concentration. These results illustrate the feasibility of using SAW as a novel method for rapidly producing particles and droplets with a controlled and narrow size distribution