Single Site Discrimination of Cytosine, 5-Methylcytosine, and 5-Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes
Single Site Discrimination of Cytosine, 5-Methylcytosine, and 5-Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes
复制标题
使用蒽标记荧光探针对目标 DNA 中的胞嘧啶、5-甲基胞嘧啶和 5-羟甲基胞嘧啶进行单位点鉴别
DOI：
10.1021/acschembio.5b00796
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发表时间：
2015
影响因子：
4
通讯作者：
Duprey J
中科院分区：
文献类型：
--
作者：
Duprey J
The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.
