A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols.
A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols.
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DOI：
10.1016/j.talanta.2013.11.065
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发表时间：
2014-02
期刊：
影响因子：
6.1
通讯作者：
Zhenzhu Wu;Wenying Li;Jian Chen;Cong Yu
中科院分区：
文献类型：
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作者：
Zhenzhu Wu;Wenying Li;Jian Chen;Cong Yu
建立了一种基于石墨烯量子点（GQD）的高灵敏度和高选择性传感生物硫醇的简便检测方法。GQD在水性缓冲溶液中发出强烈的蓝色荧光。据观察，汞（II）离子可以有效地结合和淬灭的GQD的荧光。当将生物硫醇化合物（谷胱甘肽、半胱氨酸或高半胱氨酸）加入GQD和汞（II）的测定混合物中时，它与汞（II）离子结合。Hg ~（2+）-GQD复合物解离，并检测到荧光开启信号。GQD的发射强度变化可能与加入试验溶液中的生物硫醇量直接相关。该方法灵敏度高，GSH、Cys和Hcy的检出限分别为5 nM、2.5 nM和5 nM。该测定法也具有高度选择性，检测了许多氨基酸和蛋白质，几乎没有观察到干扰。此外，还证明了血清样品中GSH标准品的回收率。我们设想，我们的测定方法可以促进生物硫醇定量相关的生物和生物医学研究。
A facile assay method for the highly sensitive and selective sensing of biothiols based on graphene quantum dots (GQDs) has been developed. GQDs emitted strong blue fluorescence in an aqueous buffer solution. It was observed that mercury(II) ions could efficiently bind and quench the fluorescence of the GQDs. When a biothiol compound (glutathione, cysteine, or homocysteine) was added to the assay mixture of GQDs and mercury(II), it bound to mercury(II) ions. Hg2+–GQD complex dissociated, and a fluorescence turn-on signal was detected. The emission intensity changes of the GQDs could be directly related to the amount of biothiol added to the assay solution. The assay is highly sensitive, the limits of detection (LOD) for GSH, Cys and Hcy were 5 nM, 2.5 nM and 5 nM, respectively. The assay is also highly selective, a number of amino acids and proteins were tested, and little interference was observed. In addition, GSH standard recovery in serum samples was also demonstrated. We envision that our assay method could facilitate the biothiol quantification related biological and biomedical research.