Measurement of mitochondrial oxygen consumption using a Clark electrode.
Measurement of mitochondrial oxygen consumption using a Clark electrode.
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DOI：
10.1007/978-1-61779-504-6_5
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Graham BH
中科院分区：
文献类型：
--
作者：
Li Z;Graham BH
Mitochondria require oxygen to produce ATP in sufficient quantities to drive energy-requiring reactions in eukaryotic organisms. The measurement of oxygen consumption rates from isolated mitochondria in vitro is a useful and valuable technique in the research and evaluation of mitochondrial dysfunction and disease since ADP-dependent oxygen consumption directly reflects coupled respiration or oxidative phosphorylation (OXPHOS). This chapter describes the traditional method of mitochondrial polarography using a Clark electrode for measuring coupled respiration in freshly isolated mitochondria from both mammalian tissues and Drosophila melanogaster.
