A novel ompC mutation of Escherichia coli K-12 that reduces OmpC and OmpF levels in the outer membrane.
A novel ompC mutation of Escherichia coli K-12 that reduces OmpC and OmpF levels in the outer membrane.
复制标题
大肠杆菌 K-12 的一种新型 ompC 突变可降低外膜中的 OmpC 和 OmpF 水平。
DOI：
10.1111/j.1365-2958.1993.tb00973.x
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发表时间：
1993
影响因子：
3.6
通讯作者：
Misra,R
中科院分区：
文献类型：
--
作者：
Misra,R
A novelompCmutation was isolated that not only lowered the amount of its own product, OmpC27, but also reduced the level of OmpF present in the outer membrane.ompC27codes for a mutant OmpC protein that contains two non‐native cysteine residues. TheompC27allele confers phage resistance by lowering the level of OmpC present in the outer membrane. This effect on OmpC27 was manifested at the level of assembly as a result of disulphide bond formation between the two cysteine residues. This disulphide bonding in OmpC27 also produced a novel phenotype by specifically influencing OmpF levels. The effect of OmpC27 on OmpF was partly a result of a lowering ofompFtranscription, and partly a result of an effect at the post‐transcription level. The transcriptional effect is likely to be brought about by a defective membrane as a result of the insertion of the disulphide bond containing OmpC27. The post‐transcriptional effect of OmpC27 on OmpF could be due to interference at the assembly level. In adsbA::kan1background where thein vivodisulphide bonding ability was dramatically reduced, the OmpC27‐mediated effects were also curtailed.
