Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer.
Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer.
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DOI：
10.1016/j.foodchem.2014.01.001
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发表时间：
2014-07
期刊：
影响因子：
8.8
通讯作者：
Qinxin Song;Guijiang Wei;Guohua Zhou
中科院分区：
文献类型：
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作者：
Qinxin Song;Guijiang Wei;Guohua Zhou
利用光电二极管(PD)阵列，研制了一种用于转基因生物DNA序列检测的便携式生物发光分析仪。成功地对8个基因(转基因玉米的zSSIIb、Bt11和Bt176基因；转基因抗草甘膦大豆的凝集素、35S-CTP4、CP4EPSPS、CaMV35S启动子和NOS终止子)进行了焦磷酸测序。相应的检测下限(LOD)为0.01%，共35次。对来自中国三个不同种源的玉米和大豆进行了检测。结果表明，使用小尺寸的检测器进行焦磷酸测序是一种简单、廉价和可靠的转基因分析农场/田间试验方法。
A portable bioluminescence analyzer for detecting the DNA sequence of genetically modified organisms (GMO) was developed by using a photodiode (PD) array. Pyrosequencing on eight genes (zSSIIb, Bt11, and Bt176 gene of genetically modified maize; Lectin, 35S-CTP4, CP4EPSPS, CaMV35S promoter, and NOS terminator of the genetically modified roundup ready soya) was successfully detected with this instrument. The corresponding limit of detection (LOD) was 0.01 % with 35 PCR cycles. The maize and soya available from three different provenances in China were detected. The results indicate that pyrosequencing using the small size of the detector is a simple, inexpensive, and reliable way in a farm/field test of GMO analysis.