An angiotensin II AT1 receptor antagonist, telmisartan augments glucose uptake and GLUT4 protein expression in 3T3-L1 adipocytes
An angiotensin II AT1 receptor antagonist, telmisartan augments glucose uptake and GLUT4 protein expression in 3T3-L1 adipocytes
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DOI：
10.1016/j.febslet.2004.09.027
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发表时间：
2004-10-22
期刊：
影响因子：
3.5
通讯作者：
Nakao, K
中科院分区：
文献类型：
--
作者：
Fujimoto, M;Masuzaki, H;Nakao, K
越来越多的证据表明，一些血管紧张素 II AT(1) 受体拮抗剂具有胰岛素增敏特性。因此，我们使用 3T3-L1 脂肪细胞检查了替米沙坦对胰岛素作用的影响。使用标准分化诱导剂，较高剂量的替米沙坦有效促进 3T3-L1 前脂肪细胞的分化。用替米沙坦处理分化脂肪细胞和完全分化脂肪细胞会导致 PPARγ 靶基因（例如 aP2 和脂联素）的 mRNA 水平呈剂量依赖性增加。相比之下，替米沙坦减弱了分化脂肪细胞中 11β-羟基类固醇脱氢酶 1 型 mRNA 水平。值得注意的是，我们首次证明替米沙坦在脂肪细胞的基础状态和胰岛素刺激状态下均增强了 GLUT4 蛋白表达和 2-脱氧葡萄糖摄取，这可能至少部分有助于其胰岛素增敏能力。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Evidence has accumulated that some of the angiotensin II AT(1) receptor antagonists have insulin-sensitizing property. We thus examined the effect of telmisartan on insulin action using 3T3-L1 adipocytes. With standard differentiation inducers, a higher dose of telmisartan effectively facilitated differentiation of 3T3-L1 preadipocytes. Treatment of both differentiating adipocytes and fully differentiated adipocytes with telmisartan caused a dose-dependent increase in mRNA levels for PPARgamma target genes such as aP2 and adiponectin. By contrast, telmisartan attenuated 11beta-hydroxysteroid dehydrogenase type 1 mRNA level in differentiated adipocytes. Of note, we demonstrated for the first time that telmisartan augmented GLUT4 protein expression and 2-deoxy glucose uptake both in basal and insulin-stimulated state of adipocytes, which may contribute, at least partly, to its insulin-sensitizing ability. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.