A novel negative-stranded RNA virus mediates sex ratio in its parasitoid host.
A novel negative-stranded RNA virus mediates sex ratio in its parasitoid host.
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一种新型负链RNA病毒介导其寄生宿主的性别比例
DOI：
10.1371/journal.ppat.1006201
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发表时间：
2017-03
期刊：
影响因子：
6.7
通讯作者：
Ye G
中科院分区：
文献类型：
--
作者：
Wang F;Fang Q;Wang B;Yan Z;Hong J;Bao Y;Kuhn JH;Werren JH;Song Q;Ye G
寄生蜂是自然界和农业生态系统中节肢动物的重要天敌，经常与病毒或病毒粒子相关联。在这里，我们报告了一种新的负链RNA病毒从寄生蜂（Pteromalus puparum）。完整的病毒基因组长度为12，230个核苷酸，含有5个非重叠的线性排列的开放阅读框。系统发生学上，该病毒与Nyamiviridae单负链RNA病毒科的新成员聚簇，在此被命名为Pteromalus puparum负链RNA病毒1（PpNSRV-1）。PpNSRV-1存在于寄生蜂的各个组织和生命阶段，并通过感染的雌性和雄性垂直传播。田间种群的病毒感染率为16.7 ~ 37.5%，与温度无线性关系。PpNSRV-1增加了成虫寿命，并损害了几个适合度参数的黄蜂，但没有影响成功的寄生。引人注目的是，PpNSRV-1介导的后代性别比减少雌性后代的数量。RNA干扰敲除病毒开放阅读框I消除了这些PpNSRV-1诱导的效应。因此，我们推断，PpNSRV-1有复杂的影响，其昆虫宿主，包括性别比例扭曲男性，以及可能的互利互惠的好处，通过增加黄蜂寿命。
Parasitoid wasps are important natural enemies of arthropod hosts in natural and agricultural ecosystems and are often associated with viruses or virion-like particles. Here, we report a novel negative-stranded RNA virus from a parasitoid wasp (Pteromalus puparum). The complete viral genome is 12,230 nucleotides in length, containing five non-overlapping, linearly arranged open reading frames. Phylogenetically, the virus clusters with and is a novel member of the mononegaviral family Nyamiviridae, here designated as Pteromalus puparum negative-strand RNA virus 1 (PpNSRV-1). PpNSRV-1 is present in various tissues and life stages of the parasitoid wasp, and is transmitted vertically through infected females and males. Virus infections in field populations of P. puparum wasps ranged from 16.7 to 37.5%, without linearly correlating with temperature. PpNSRV-1 increased adult longevity and impaired several fitness parameters of the wasp, but had no influence on successful parasitism. Strikingly, PpNSRV-1 mediated the offspring sex ratio by decreasing female offspring numbers. RNA interference knockdown of virus open reading frame I eliminated these PpNSRV-1-induced effects. Thus, we infer that PpNSRV-1 has complex effects on its insect host including sex ratio distortion towards males, as well as possible mutualistic benefits through increasing wasp longevity.