Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant
Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant
复制标题
DOI：
10.1111/j.1365-2958.2004.04154.x
复制
发表时间：
2004-07-01
影响因子：
3.6
通讯作者：
Schoolnik, GK
中科院分区：
文献类型：
--
作者：
Yildiz, FH;Liu, XS;Schoolnik, GK
预计有皱纹和光滑菌落变体之间的可逆相变对于霍乱弧菌在自然水生栖息地中的生存很重要。对同一菌株的皱纹和光滑变体进行的微阵列表达谱研究鉴定出 124 个差异调节基因。进一步的表达谱实验显示了这些基因如何受到 VpsR 和 HapR 转录因子的调节，它们分别正向和负向调节 VPSEl Tor（一种与皱纹相关的胞外多糖）的产生。 rpoN 和 rpoS 突变体的研究证明了这些替代 sigma 因子对相位变化特异性基因表达的影响。对这些表达数据的生物信息学分析表明，“粗糙度”和“平滑度”是由正负调节因子的复杂层次结构决定的，这也影响生物体的生物膜、表面疏水性和运动表型。
Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identification of 124 differentially regulated genes. Further expression profiling experiments showed how these genes are regulated by the VpsR and HapR transcription factors, which, respectively, positively and negatively regulate production of VPSEl Tor, a rugose-associated extracellular polysaccharide. The study of mutants of rpoN and rpoS demonstrated the effects of these alternative sigma factors on phase variation-specific gene expression. Bioinformatics analysis of these expression data shows that 'rugosity' and 'smoothness' are determined by a complex hierarchy of positive and negative regulators, which also affect the biofilm, surface hydrophobicity and motility phenotypes of the organism.