Overexpression of CCT8 and its significance for tumor cell proliferation, migration and invasion in glioma
Overexpression of CCT8 and its significance for tumor cell proliferation, migration and invasion in glioma
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CCT8在胶质瘤中的过表达及其对肿瘤细胞增殖、迁移和侵袭的意义
DOI：
10.1016/j.prp.2015.04.012
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发表时间：
2015-01-01
影响因子：
2.8
通讯作者：
Wang, Donglin
中科院分区：
文献类型：
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作者：
Qiu, Xiaojun;He, Xiaojuan;Wang, Donglin
在各种类型的恶性肿瘤中已经报道了含有t-复合物多肽1（TCP 1）或CCT的伴侣蛋白的过表达。然而，很少有人知道的t-复合体蛋白亚基TCP 1 θ（CCT 8）在胶质瘤中的表达。本研究采用免疫印迹法和免疫组化法检测了CCT 8蛋白的表达。发现CCT 8在神经胶质瘤中过表达，并且与神经胶质瘤的WHO分级相关。为了进一步研究CCT 8在胶质瘤中的生物学功能，使用小干扰RNA（siRNA）序列构建CCT 8沉默的U87多形性胶质母细胞瘤（GBM）和U251 MG细胞。使用CCK 8、流式细胞术循环、划痕、transwell侵袭和荧光测定来分析CCT 8对这些细胞中的增殖和侵袭的敲低效应。与对照组相比，表达CCT 8-siRNA的胶质瘤细胞表现出显著降低的增殖和侵袭能力，以及细胞骨架失调。本研究表明，CCT 8蛋白的高表达可能与胶质瘤的不良预后有关，并且CCT 8调节胶质母细胞瘤的增殖和侵袭。(C)2015 Elsevier GmbH. All rights reserved.
Overexpression of chaperonin containing t-complex polypeptide 1 (TCP1), or CCT, has been reported in various classes of malignancies. However, little is known about the expression of t-complex protein subunits TCP1theta (CCT8) in gliomas. In this study, the expression of CCT8 protein was detected using blotting analysis and immunohistochemistry. CCT8 was found to be overexpressed in gliomas and to correlate with the WHO grade of gliomas. To further investigate the biological function of CCT8 in gliomas, CCT8-silenced U87 glioblastoma multiforme (GBM) and U251MG cells were constructed using a small interference RNA (siRNA) sequence. The knockdown effect of CCT8 on proliferation and invasion in these cells was analyzed using the CCK8, flow cytometry cycle, scratch, transwell invasion and fluorescence assays. Compared with the controls, the glioma cells expressing CCT8-siRNA exhibited a significantly decreased proliferation and invasion capacity, as well as a dysregulated cell cytoskeleton. This study showed that high CCT8 protein expression might be related to poor outcome of glioma, and that CCT8 regulates the proliferation and invasion of glioblastomas. (C) 2015 Elsevier GmbH. All rights reserved.