Elevated TGF-β1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer
Elevated TGF-β1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer
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DOI：
10.1007/s00005-006-0016-0
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发表时间：
2006-03-01
影响因子：
3.2
通讯作者：
Chazan, R
中科院分区：
文献类型：
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作者：
Dornagafa-Kulawik, J;Hoser, G;Chazan, R
转化生长因子（TGF）-β是由癌细胞产生的调节抗肿瘤免疫的众多抑制因子之一。本研究的目的是评估原发性肺癌患者支气管肺泡灌洗液（BALF）中TGF-β 1水平和淋巴细胞亚群，并分析这些参数之间的相互依赖性。材料和方法：收集38例原发性肺癌患者治疗前和23例健康志愿者的BALF样本。在浓缩上清液后，使用市售夹心ELISA试剂盒在两个独立批次的样品中测量TGF-β 1的浓度。在用May Grunwald Giemsa方法染色的载玻片上进行BALF中的差异细胞计数。应用单克隆抗体流式细胞仪进行淋巴细胞表型分析。与健康受试者相比，在两批样品中均观察到患者BALF中的总胆固醇水平（分别为3.23 +/-2.96pg/ml vs.1.05 +/-0.95pg/ml，p < 0.05，和16.1 +/-19.3pg/ml vs.10.1 +/-11.1pg/ml，差异不显著）。TGF-β 1水平与淋巴细胞比例呈显著正相关，与巨噬细胞比例、细胞毒性T淋巴细胞和活化T淋巴细胞比例呈显著负相关。
Introduction: Transforming growth factor (TGF)-beta is one of numerous inhibitory factors produced by cancer cells that regulate antitumor immunity. The aim of this study was to evaluate TGF-beta 1 levels and lymphocyte subsets in the broncholaveolar lavage fluid (BALF) of patients with primary lung cancer and to analyze the interdependence of these parameters.Materials and Methods: BALF samples were collected from 38 patients with primary lung cancer prior to treatment and from 23 healthy volunteers. Concentrations of TGF-beta 1 were measured in two independent lots of samples using a commercially available sandwich ELISA kit after concentration of the supernatants. Differential cell counts in the BALF were performed on slides stained with the May Grunwald Giemsa method. Flow cytometry with monoclonal antibodies was applied for lymphocyte phenotyping.Results: A higher level of TGF-beta 1. in the BALF of patients compared with the healthy subjects was observed in both lots of samples (3.23 +/- 2.96 pg/ml vs. 1.05 +/- 0.95 pg/ml, p < 0.05, and 16.1 +/- 19.3 pg/ml vs. 10.1 +/- 11.1 pg/m,, respectively, difference not significant). There was significant positive correlation of the TGF-beta 1 level with the proportion of lymphocytes and negative correlation with both the proportion of macrophages and the percentage of cytotoxic and activated T lymphocytes.Conclusions: Our findings confirmed that TGF-beta takes part in the local response in the course of primary lung cancer.