Transcriptional response of sugarcane roots to methyl jasmonate
Transcriptional response of sugarcane roots to methyl jasmonate
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DOI：
10.1016/j.plantsci.2004.10.006
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发表时间：
2005-03-01
期刊：
影响因子：
5.2
通讯作者：
Manners, JM
中科院分区：
文献类型：
--
作者：
Bower, NI;Casu, RE;Manners, JM
研究了茉莉酸甲酯处理对甘蔗根冠信号转导基因表达的影响。用防卫调节剂茉莉酸甲酯(MJ)处理甘蔗根部，共获得829个EST。将其中的747个EST与从甘蔗茎组织中获得的4793个EST结合在一个基因芯片中，并进行实验，以确定MJ处理24-120小时后在根中诱导的基因。使用两个数据分析系统(t-STATISTIC和TRMA)分析微阵列结果，这些方法鉴定出21个共同的EST集合，对应于MJ在根中显著诱导的转录本，以及23个在MJ处理后表达下调的EST。对微阵列分析中鉴定的6个转录本的诱导进行了测试，并用Northern blotting进行了确认。MJ处理后，脂氧合酶和PR-10蛋白编码基因的同源基因被诱导了824个，而其他4个被选择的转录本在以后的时间点被诱导。用MJ处理根后，在未处理的茎和叶组织中诱导出脂肪氧合酶同系物，而不是PR-10同系物。SA处理根后，在未经处理的组织中诱导出PR-10同源物和PR-1同源物，而不是脂氧合酶同源物。反复叶面喷施MJ对植株生长没有明显影响，但增加了根中脂肪氧合酶的转录水平，但不增加其他被测基因的转录水平。这些结果为进一步研究甘蔗根部诱导抗虫性奠定了基础。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
The effect of methyl jasmonate treatment on gene expression in sugarcane roots signalling between roots and shoots was studied. A collection of 829 ESTs were obtained from sugarcane roots treated with the defence-regulator methyl jasmonate (MJ) treatment. A subset of 747 of these were combined with 4793 sugarcane ESTs obtained from stem tissues in a cDNA microarray and experiments undertaken to identify genes that were induced in roots 24-120 h following treatment with MJ. Two data analysis systems (t-statistic and tRMA) were used to analyse the microarray results and these methods identified a common set of 21 ESTs corresponding to transcripts significantly induced by MJ in roots and 23 that were reduced in expression following MJ treatment. The induction of six transcripts identified in the microarray analysis was tested and confirmed using northern blotting. Homologues of genes encoding lipoxygenase and PR-10 proteins were induced 824 It after MJ treatment while the other four selected transcripts were induced at later time points. Following treatment of roots with MJ, the lipoxygenase homologue, but not the PR-10 homologue, was induced in untreated stem and leaf tissues. The PR-10 homologue and a PR-1 homologue, but not the lipoxygenase homologue, were induced in untreated tissues after the application of SA to roots. Repeated foliar application of MJ had no apparent effects on plant growth and was demonstrated to increase lipoxygenase transcripts in roots, but did not increase transcript levels-of other genes tested. These results lay a foundation for further studies of induced pest and disease resistance in sugarcane roots. (C) 2004 Elsevier Ireland Ltd. All rights reserved.