Epithelial defensins impair adenoviral infection: Implication for adenovirus-mediated gene therapy
Epithelial defensins impair adenoviral infection: Implication for adenovirus-mediated gene therapy
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DOI：
10.1089/10430349950018355
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发表时间：
1999-04-10
影响因子：
4.2
通讯作者：
Bargon, J
中科院分区：
文献类型：
--
作者：
Gropp, R;Frye, M;Bargon, J
上皮细胞通过产生称为防御素的小阳离子肽来积极参与宿主防御。为了更详细地研究上皮防御素的生物活性，我们在真核细胞系中表达了两种防御素：hBD-1和HD-5。防御素定位于细胞质和细胞培养基中，对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌表现出很强的杀菌活性。此外，我们的数据表明防御素的存在可以保护细胞免受腺病毒感染。 HD-5 或 hBD-1 的存在使 Av1Cf2 的感染性降低了三到五倍。这些结果表明，每当使用腺病毒将基因传递至上皮细胞时，必须将防御素视为严重障碍。
Epithelial cells have been to participate actively in host defense by producing small cationic peptides called defensins, To investigate the biological activity of epithelial defensins in more detail, we expressed two defensins, hBD-1 and HD-5, in eukaryotic cell lines. Defensins were localized in the cytoplasm and in cell culture medium and exhibited strong microbicidal activity toward Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Moreover, our data indicate that the presence of defensins protected the cells from adenoviral infection. The presence of HD-5 or hBD-1 reduced the infectivity of Av1Cf2 three- to fivefold. These results imply that defensins must be considered a serious obstacle whenever adenovirus is used to deliver genes to epithelial cells.