Sensitive and selective gas chromatography-tandem mass spectrometry method for the detection of nitrobenzene in tobacco smoke
Sensitive and selective gas chromatography-tandem mass spectrometry method for the detection of nitrobenzene in tobacco smoke
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DOI：
10.1016/j.chroma.2018.06.028
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发表时间：
2018-08-31
影响因子：
4.1
通讯作者：
Valentin-Blasini, Liza
中科院分区：
文献类型：
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作者：
Chapman, Gala M.;Bravo, Roberto;Valentin-Blasini, Liza
硝基苯是烟草烟雾中发现的一种潜在有害化合物，由于量化困难，在之前的分析中基本上被排除在外。量化香烟烟雾中的有害化合物有助于比较产品、检查设计参数对交付的影响以及帮助估计暴露量。开发了一种灵敏的高通量方法，可使用同位素稀释气相色谱-串联质谱 (ID-GC-MS/MS) 定量机器产生的主流卷烟烟雾中的硝基苯。该方法具有足够的灵敏度来测量低纳克范围内的气相硝基苯浓度，方法检测限为 418 pg/cig。对两种质量控制卷烟产品的精确估计导致相对标准偏差百分比为 11.5% 和 14.9%；对 13 种卷烟产品的产品变异性估计得出的相对标准偏差百分比范围为 2.8% 至 16.9%。据报道，根据加拿大卫生部强烈吸烟方案，15 种卷烟产品中机器产生的主流烟雾中硝基苯的含量为 18 至 38 纳克/支。由 Elsevier B.V. 出版
Nitrobenzene, a potentially harmful compound found in tobacco smoke, has been largely excluded from prior analysis due to difficulties with quantification. Quantifying harmful compounds in cigarette smoke is useful to compare products, to examine the impact of design parameters on delivery, and to help estimate exposures. A sensitive high-throughput method has been developed for quantifying nitrobenzene in machine-generated mainstream cigarette smoke using isotope dilution gas chromatography-tandem mass spectrometry (ID-GC-MS/MS). This method has sufficient sensitivity to measure vapor phase nitrobenzene concentrations in the low nanogram range, with a 418 pg/cig method limit of detection. Precision estimates from two quality control cigarette products resulted in percent relative standard deviations of 11.5% and 14.9%; product variability estimates from 13 cigarette products resulted in percent relative standard deviations ranging from 2.8% to 16.9%. Nitrobenzene in the machine-generated, mainstream smoke from 15 cigarette products are reported and range from 18 to 38 ng/cig under the Health Canada Intense smoking regimen. Published by Elsevier B.V.