Dibenzoylmethane induces cell cycle deregulation in human prostate cancer cells
Dibenzoylmethane induces cell cycle deregulation in human prostate cancer cells
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DOI：
10.1016/s0304-3835(01)00844-8
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发表时间：
2002-04-25
期刊：
影响因子：
9.7
通讯作者：
Harris, WB
中科院分区：
文献类型：
--
作者：
Jackson, KM;DeLeon, M;Harris, WB
二苯甲酰甲烷（DBM）是甘草和防晒霜的次要β-二酮成分，已在几种动物模型中显示出对化学诱导的皮肤癌和乳腺癌的抗肿瘤作用。迄今为止，还没有提出DBM对前列腺癌细胞生长抑制作用的机制。在这项研究中，我们研究了DBM对几种人前列腺癌细胞系的生长和细胞周期动力学的影响。使用MTT细胞毒性测定，在72 μ M暴露于DBM后观察到25-100 μ M的IC 50值。LNCaP、DU 145和PC-3前列腺癌细胞系与单独使用载体的细胞相比特别敏感。流式细胞仪分析显示，细胞周期的失调，这与观察到的细胞生长抑制作用的DBM在前列腺癌细胞。这些数据表明DBM在预防和治疗前列腺癌中的潜在作用。出版社：Elsevier Science爱尔兰有限公司
Dibenzoylmethane (DBM), a minor beta-diketone constituent of licorice and sunscreens, has been shown to exhibit anti-neoplastic effects in chemically induced skin and mammary cancers in several animal models. To date, no mechanism for the growth inhibitory effects of DBM on prostate cancer cells has been proposed, In this study, we examined the effects of DBM on the growth and cell cycle kinetics of several human prostate carcinoma cell lines. Using an MTT cytotoxicity assay, IC50 values of 25-100 muM were observed following 72 It exposure to DBM. LNCaP, DU145, and PC-3 prostate carcinoma cell lines were particularly sensitive in comparison to the cells with the vehicle alone. Flow cytometric analyses showed deregulation of the cell cycle, which correlated with the observed cytostatic effects of DBM in prostate carcinoma cells. These data suggest a potential role for DBM in the prevention and treatment of prostate cancer. Published by Elsevier Science Ireland Ltd.