Granulocyte-macrophage colony-stimulating factor stimulates human monocyte accessory cell function.
Granulocyte-macrophage colony-stimulating factor stimulates human monocyte accessory cell function.
复制标题
粒细胞巨噬细胞集落刺激因子刺激人单核细胞辅助细胞功能。
作者：
P. D. Smith;C. Lamerson;H. Wong;L. Wahl;S. Wahl
本文研究了重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）对纯化的人单核细胞辅助细胞功能的影响。与未处理的单核细胞相比，rhGM-CSF处理的单核细胞促进增强的有丝分裂原和Ag刺激的淋巴细胞增殖。抗rhGM-CSF单克隆抗体可明显抑制这种增强作用。在旨在确定rhGM-CSF增强辅助细胞功能的机制的实验中，rhGM-CSF显示出引起单核细胞表面HLA-DR分子表达的剂量依赖性增加并刺激IL-1的分泌，这两者在单核细胞T细胞相互作用中都是重要的。进一步的研究表明，rhGM-CSF可增加HLA-DR和IL-1的mRNA水平，表明HLA-DR和IL-1基因表达的转录调节。因此，rhGM-CSF增强人单核细胞的辅助细胞功能，并且这种增强与rhGM-CSF诱导的HLA-DR和IL-1基因转录的增加相关，导致表面HLA-DR表达增加和IL-1分泌增加。
We investigated the effect of recombinant human granulocyte-macrophage CSF (rhGM-CSF) on the accessory cell function of purified human monocytes. Compared with untreated monocytes, rhGM-CSF-treated monocytes promoted enhanced mitogen- and Ag-stimulated lymphocyte proliferation. This enhancement was significantly inhibited by mAb to rhGM-CSF. In experiments designed to define the mechanism of rhGM-CSF augmentation of accessory cell function, rhGM-CSF was shown to cause a dose-dependent increase in monocyte expression of surface HLA-DR molecules and stimulated secretion of IL-1, both important in monocyte T cell interactions. Further studies demonstrated that levels of HLA-DR and IL-1 mRNA were increased by rhGM-CSF, indicating transcriptional regulation of gene expression for HLA-DR and IL-1. Thus, rhGM-CSF augments accessory cell function by human monocytes, and this augmentation correlates with rhGM-CSF-induced increases in transcription of the HLA-DR and IL-1 genes leading to increased expression of surface HLA-DR and secretion of IL-1.