Microbial degradation of poly(D-3-hydroxybutyrate) by a new thermophilic Streptomyces isolate
Microbial degradation of poly(D-3-hydroxybutyrate) by a new thermophilic Streptomyces isolate
复制标题
DOI：
10.1023/b:bile.0000009453.81444.51
复制
发表时间：
2004-01-01
影响因子：
2.7
通讯作者：
Tokiwa, Y
中科院分区：
文献类型：
--
作者：
Calabia, BP;Tokiwa, Y
从土壤中分离到一种新的能降解聚d -3-羟基丁酸酯（PHB）的嗜热微生物。基于16S rDNA序列的系统发育分析表明，该分离株属于链霉菌属。在50℃下培养6 d后，PHB膜和PHB粉完全降解。扫描显微照片显示，微生物细胞粘附在整个膜表面，表明生物降解是通过PHB表面的定植发生的。通过微生物的攻击和微生物分泌的胞外酶的作用，膜被降解。菌株也能降解聚琥珀酸乙酯、聚碳酸酯、聚己内酯和聚琥珀酸丁二烯，但降解程度较低。
A new thermophilic microorganism capable of degrading poly(D-3-hydroxybutyrate) (PHB) was isolated from soil. A phylogenetic analysis based on 16S rDNA sequences indicated that the new isolate belongs to genus Streptomyces. PHB film and powder were completely degraded after 6 and 3 d cultivation, respectively at 50degreesC. Scanning micrographs showed adherence of the microbial cells to the entire film surface, indicating that biodegradation occurs by colonization of the PHB surface. The film was degraded both by microbial attack and by the action of an extracellular enzyme secreted by the microorganism. The strain can also degrade poly( ethylene succinate), poly( ester carbonate), polycaprolactone and poly( butylene succinate), but to a lesser extent.