Selective molecular assembly patterning: A new approach to micro- and nanochemical patterning of surfaces for biological applications
Selective molecular assembly patterning: A new approach to micro- and nanochemical patterning of surfaces for biological applications
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DOI：
10.1021/la011715y
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发表时间：
2002-04-16
期刊：
影响因子：
3.9
通讯作者：
Spencer, ND
中科院分区：
文献类型：
--
作者：
Michel, R;Lussi, JW;Spencer, ND
提出了一种基于烷基磷酸盐在金属氧化物表面选择性自组装的新型图案化技术。标准光刻被用来在二氧化硅的基质中产生二氧化钛的图案。烷基磷酸盐可在二氧化钛表面自组装，但不能在二氧化硅表面自组装。随后对聚(L-赖氨酸)-g-聚乙二醇(PLL-g-PEG)的吸附使暴露的二氧化硅表面对蛋白质的吸附产生抵抗。利用X射线光电子能谱和飞行时间二次离子质谱仪对组装过程进行了监测。通过荧光显微镜对蛋白质的吸附研究，最终确定了所得表面具有蛋白质粘附性，烷基磷酸盐修饰的二氧化钛的特征，排列在蛋白质抗性的PLL-g-PEG改性的二氧化硅基质中。在含血清的培养液中孵育的人包皮成纤维细胞被发现选择性地附着在蛋白质粘附区，在那里它们形成了局灶性接触。在至少14天内，细胞与PLL-g-PEG包被的二氧化硅区域没有明显的相互作用。这种图案化方法被称为选择性分子组装图案化，被认为适合于在大范围内重复性和成本效益地制造与生物相关的化学图案。
A novel patterning technique based on selective self-assembly of alkane phosphates on metal oxide surfaces is presented. Standard photolithography was used to create patterns of titanium dioxide within a matrix of silicon dioxide. Alkane phosphates were found to self-assemble on TiO2 but not on SiO2, surfaces. Subsequent adsorption of poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) rendered the exposed SiO2 surface resistant to protein adsorption. X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry were employed to monitor the assembly processes. Protein-adsorption studies by means of fluorescence microscopy conclusively established that the resulting surfaces displayed protein-adhesive, alkyl phosphate modified TiO2 features, arranged within a protein-resistant PLL-g-PEG-modified SiO2 matrix. Human foreskin fibroblasts, incubated in a serum-containing medium, were found to selectively attach to the protein-adhesive areas, where they developed focal contacts. No interaction of cells with the PLL-g-PEG-coated SiO2 areas was evident for at least 14 days. This patterning approach, termed selective molecular assembly patterning, is considered to be suitable for reproducible and cost-effective fabrication of biologically relevant chemical patterns over large areas.