Lactobacillus plantarum Prevents Bacterial Translocation in Rats Following Ischemia and Reperfusion Injury
Lactobacillus plantarum Prevents Bacterial Translocation in Rats Following Ischemia and Reperfusion Injury
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植物乳杆菌防止大鼠缺血和再灌注损伤后的细菌移位
DOI：
10.1007/s10620-011-1747-2
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发表时间：
2011-11-01
影响因子：
3.1
通讯作者：
Li, Jieshou
中科院分区：
文献类型：
--
作者：
Wang, Bin;Huang, Qian;Li, Jieshou
背景：细菌易位被认为是临床上引起全身性败血症和多器官功能障碍的主要原因。本研究的目的是确定一种植物乳杆菌预防缺血/再灌注（I/R）诱导的肠道感染的能力。方法：雌性Sprague-Dawley大鼠随机分为3组：(1)对照组（假手术，未处理）、(2)缺血再灌注组和(3)缺血再灌注和植物乳杆菌处理组。在I/R诱导前14天，每天灌胃植物乳杆菌L2。然后处死大鼠，培养组织和血液样本以确定细菌易位。ELISA法检测血浆细胞因子。取回肠段进行形态学检查。结果：肠道I/R诱导肠道内过度的促炎细胞因子分泌和屏障功能障碍（上皮细胞凋亡增加、盲肠菌群失调、黏膜破坏和多发性糜坏），与肠道外细菌易位增加有关。大约87.5%暴露于I/R的大鼠发生细菌易位，而对照组没有细菌易位。然而，植物乳杆菌预处理动物完全阻止了I/R诱导的细菌易位，减少了促炎细胞因子的释放和肠上皮细胞的凋亡，从而恢复了微生物群和粘膜的完整性。结论：植物乳杆菌L2可以预防I/ r诱导的细菌易位和肠道屏障功能障碍，从而在肠道中发挥有益作用。
Background:Bacterial translocation is considered a major cause of initiation and development of systemic sepsis and multiple organ dysfunction in clinic. The aim of this study was to determine the ability of a defined Lactobacillus plantarum to prevent ischemia/reperfusion (I/R) induced intestinal infection.Methods:Female Sprague-Dawley rats were randomly allocated into three groups: (1) controls (sham-operated, no treatment), (2) ischemia/reperfusion and (3) ischemia/reperfusion and Lactobacillus plantarum treatment. Lactobacillus plantarum L2 was administered daily intragastrically 14 days prior to induction of I/R. Rats were then sacrificed, and tissue and blood samples were cultured to determine bacterial translocation. Cytokines in plasma were detected by ELISA. Ileal segments were removed for morphological examination.Results:Intestinal I/R induced excess pro-inflammatory cytokine secretion and barrier dysfunction (increased epithelial cell apoptosis, cecal flora dysbiosis, disruption of mucosa and multiple erosions) in the intestine, associated with increased bacterial translocation to extraintestinal sites. Approximately 87.5% of rats exposed to I/R had bacterial translocation while there was no bacterial translocation in controls. However, pretreatment of animals with Lactobacillus plantarum completely prevented I/R induced bacterial translocation, reduced pro-inflammatory cytokine release, and intestinal epithelial cell apoptosis, resulting in recovered microflora and mucosal integrity.Conclusions:These findings indicate that Lactobacillus plantarum L2 can prevent I/R-induced bacterial translocation and intestinal barrier dysfunction and, thereby, exert beneficial effects in the intestinal tract.