Influence of growth factors on tissue-engineered pediatric elastic cartilage.
Influence of growth factors on tissue-engineered pediatric elastic cartilage.
复制标题
生长因子对组织工程儿童弹性软骨的影响。
DOI：
10.1001/archotol.126.10.1234
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
R. Eavey
中科院分区：
文献类型：
--
作者：
C. A. Arévalo;Y. Cao;M. Vacanti;Y. Weng;C. Vacanti;R. Eavey
目的
探讨生长因子对组织工程化儿童弹性软骨的影响及其临床应用前景。
设计
对照研究。
科目
11名年龄在5至15岁之间的儿童提供了耳廓弹性软骨标本，尺寸约为1 x 1 x 0.2 cm，重量约为100 mg。
干预措施
将300万个软骨细胞接种到4组Ham F-12培养基中：组1，仅Ham F-12培养基;无生长因子（对照组）;组2，Ham F-12培养基和碱性成纤维细胞生长因子;组3，Ham F-12培养基和转化生长因子β;以及组4，Ham F-12培养基和两种生长因子的组合。在3周时，收获细胞并与聚乙二醇和聚环氧丙烷的共聚物凝胶（Pluronic F-127）混合。将细胞溶液皮下注射到无胸腺小鼠中。在长达22周的体内培养时收获构建体并进行组织学分析。
结果
体外产生的平均细胞数如下：第1组，1200万;第2组，4000万;第3组，700万;第4组，3500万。第2组体内大体标本最大且最重。在组织学上，对照组和碱性成纤维细胞生长因子组（第1组和第2组）表现出与正常耳软骨相容的特征;第3组和第4组表现出细胞解体和中度至重度纤维组织浸润。
结论
碱性成纤维细胞生长因子对工程化小儿耳廓软骨的体外和体内生长具有最大的积极影响。结果表明，碱性成纤维细胞生长因子具有临床应用的潜力，其目标是在短时间内从小供体标本中产生大量的组织工程化软骨，其质量类似于天然人弹性软骨。
OBJECTIVE
To investigate the influence of growth factors on tissue-engineered pediatric human elastic cartilage relative to potential clinical application.
DESIGN
Controlled study.
SUBJECTS
Eleven children ranging in age from 5 to 15 years provided auricular elastic cartilage specimens measuring approximately 1 x 1 x 0.2 cm and weighing approximately 100 mg.
INTERVENTIONS
Three million chondrocytes were plated into 4 groups of Ham F-12 culture medium: group 1, Ham F-12 culture medium only; no growth factors (control group); group 2, Ham F-12 culture medium and basic fibroblast growth factor; group 3, Ham F-12 culture medium and transforming growth actor beta; and group 4, Ham F-12 culture medium and a combination of both growth factors. At 3 weeks, the cells were harvested and mixed with a copolymer gel of polyethylene glycol and polypropylene oxide (Pluronic F-127). The cell solution was injected subcutaneously into athymic mice. The constructs were harvested at up to 22 weeks of in vivo culture and histologically analyzed.
RESULTS
The average number of cells generated in vitro was as follows: group 1, 12 million; group 2, 40 million; group 3, 7 million; and group 4, 35 million. Group 2 in vivo gross specimens were the largest and heaviest. Histologically, the control group and the basic fibroblast growth factor group (groups 1 and 2) exhibited characteristics compatible with normal auricular cartilage; groups 3 and 4 demonstrated cellular disorganization and moderate to severe fibrous tissue infiltration.
CONCLUSIONS
Basic fibroblast growth factor demonstrates the greatest positive influence on the in vitro and in vivo growth of engineered pediatric human auricular cartilage. The results suggest that basic fibroblast growth factor has the potential for clinical application in which a goal will be to generate a large volume of tissue-engineered cartilage from a small donor specimen in a short period of time and of a quality similar to native human elastic cartilage.
小仓鼠肾细胞成纤维细胞生长因子受体的鉴定和部分表征。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Neufeld,G;Gospodarowicz,D
通讯作者：
Gospodarowicz,D