Antimicrobial susceptibility of Helicobacter pylori strains isolated from patients in Shiraz, Southern Iran
Antimicrobial susceptibility of Helicobacter pylori strains isolated from patients in Shiraz, Southern Iran
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DOI：
10.3748/wjg.v16.i45.5746
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发表时间：
2010-12-07
影响因子：
4.3
通讯作者：
Hosseini, Marziyeh
中科院分区：
文献类型：
--
作者：
Farshad, Shohreh;Alborzi, Abdolvahab;Hosseini, Marziyeh
目的：了解伊朗幽门螺杆菌(Hp)耐药率的地区差异，寻找根除Hp感染的最佳抗生素治疗方法。方法：对伊朗南部设拉子市266例接受胃镜检查的患者进行研究。从胃窦活检标本中分离出幽门螺杆菌菌株，并进行快速尿素酶试验和革兰氏染色鉴定。用E-test法测定幽门螺杆菌对抗生素的敏感性。结果：共分离出Hp菌株121株，其中男性50株，女性71株。对甲硝唑、阿莫西林、克拉霉素和四环素的耐药率分别为44%(n=53)、20%(n=24)、5%(n=6)和3%(n=4)。当同时考虑抗生素时，我们发现了11种菌株的敏感模式。女性患者对甲硝唑的耐药率明显高于男性患者(P<；0.05)。约71%的甲硝唑耐药株对甲硝唑的最低抑菌浓度(MIC)>256ug/ml。结论：甲硝唑原发耐药率中等。然而，71%的分离株具有较高的MIC(~gt；256 mg/L)。在阿莫西林的案例中，20%的耐药率上升令人担忧。我们地区的幽门螺杆菌菌株也出现了对克拉霉素和四环素的耐药性。(C)2010年白石登。版权所有。
AIM: To improve our understanding of Iranian regional variation in Helicobacter pylori (H. pylon) antibiotic resistance rates to find the best antibiotic therapy for eradication of H. pylori infections.METHODS: A total of 266 patients undergoing endoscopy in Shiraz, Southern Iran, were included in this study. H. pylori strains were isolated from antral biopsies by culture and confirmed by the rapid urease-test and gram staining. Antibiotic susceptibility of H. pylon isolates was determined by E-test. RESULTS: A total of 121 H. pylori strains were isolated, 50 from male and 71 from female patients. Data showed that 44% (n = 53), 20% (n = 24), 5% (n = 6), and 3% (n = 4) of all strains were resistant to the antibiotics metronidazole, amoxicillin, clarithromycin, and tetracycline, respectively. When the antibiotics were considered together we found 11 sensitivity patterns for the strains. Resistance to metronidazole was significantly higher in female than in male patients (P < 0.05). In about 71% of the metronidazole-resistant isolates, the minimum inhibitory concentrations (MICs) exceeded 256 mu g/mL.CONCLUSION: We found a moderate rate of primary resistance to metronidazole. However, a high MIC (> 256 mg/L) which was found in 71% of the isolates is considerable. In the case of amoxicillin, an increased resistance rate of 20% is worrying. Resistance to clarithromycin and tetracycline is also emerging among the H. pylori strains in our region. (C) 2010 Baishideng. All rights reserved.