Human adipose stromal cells expanded in human serum promote engraftment of human peripheral blood hematopoietic stem cells in NOD/SCID mice
Human adipose stromal cells expanded in human serum promote engraftment of human peripheral blood hematopoietic stem cells in NOD/SCID mice
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DOI：
10.1016/j.bbrc.2005.01.092
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Jung, JS
中科院分区：
文献类型：
--
作者：
Kim, SJ;Cho, HH;Jung, JS
据报道，人间充质干细胞 (hMSC) 存在于骨髓、脂肪组织、真皮、肌肉和外周血中，具有沿不同谱系分化的潜力，包括形成骨、软骨、脂肪、肌肉和神经元的谱系。因此，hMSC 是细胞和基因治疗的有吸引力的候选者。hMSC 扩增的最佳条件需要添加胎牛的培养基 血清（胎牛血清）。某些形式的细胞疗法将涉及多次剂量，引起人们对培养基衍生的胎牛血清蛋白引起的免疫反应的担忧。在这项研究中，我们在分离和扩增过程中在人血清（HS）中培养了人脂肪基质细胞（hADSC）和骨髓基质细胞（HBMSC），并证明了，，；维持其增殖能力和多谱系分化能力并促进 从骨髓中动员的外周血来源的 CD34(+) 细胞植入 NOD/SCID 小鼠体内。我们的结果表明，在 HS 中培养的 hADSC 和 hBMSC 可用于细胞和基因治疗的临床试验，包括促进同种异体 HSC 移植后的植入。 (C) 2005 Elsevier Inc. 保留所有权利。
Human mesenchymal stem cells (hMSC, that have been reported to be present in bone marrow, adipose tissues, dermis, muscles, and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle, and neuron. Therefore, hMSC are attractive candidates for cell and gene therapy. The optimal conditions for hMSC expansion require medium supplemented with fetal bovine serum (FBS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FBS proteins. In this study, we cultured human adipose stromal cells (hADSC) and bone marrow stroma cells (HBMSC) in human serum (HS) during their isolation and expansion, and demonstrated that the,,; maintain their proliferative capacity and ability for multilineage differentiation and promote engraftment of peripheral blood-derived CD34(+) cells mobilized from bone marrow in NOD/SCID mice. Our results indicate that hADSC and hBMSC cultured in HS can be used for clinical trials of cell and gene therapies, including promotion of engraftment after allogeneic HSC transplantation. (C) 2005 Elsevier Inc. All rights reserved.