CD83 expression is a sensitive marker of activation required for B cell and CD4+ T cell longevity in vivo
CD83 expression is a sensitive marker of activation required for B cell and CD4+ T cell longevity in vivo
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DOI：
10.4049/jimmunol.179.7.4550
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发表时间：
2007-10-01
影响因子：
4.4
通讯作者：
Tedder, Thomas F.
中科院分区：
文献类型：
--
作者：
Prazma, Charlene M.;Yazawa, Norihito;Tedder, Thomas F.
CD83是区分未成熟和成熟人类树突状细胞群体的表面标志。胸腺上皮细胞CD83的表达也是小鼠有效发育CD4(+)T细胞所必需的。CD83(-/-)小鼠外周B细胞和CD4(+)T细胞表型的改变，以及外周CD4(+)T细胞的减少，表明CD83具有额外的功能。为了评估这一点，产生了一组单抗来表征外周血白细胞对小鼠CD83的表达。与人类一样，常规和浆细胞样小鼠树突状细胞亚群的激活导致小鼠CD83表面表达迅速上调。在初级和次级淋巴室中，B细胞亚群低水平表达CD83，而静息T细胞上未检测到CD83。然而，CD83在体外激活后的几小时内显著上调大多数脾B细胞和T细胞。在体内，低剂量的鸡蛋溶菌酶(1mUg)在抗原攻击后4h内可显著诱导抗原特异性B细胞表达CD83，但不能诱导CD69表达。虽然CD83(-/-)小鼠的B细胞发育正常，但在过继转移实验中，CD83的B和CD4(+)T细胞的表达是淋巴细胞存活所必需的。因此，CD83的限制性表达模式，它在B细胞和T细胞激活后的快速诱导，以及它对B细胞和CD4(+)T细胞寿命的要求，表明CD83是体内早期淋巴细胞激活的一个重要的功能和敏感的标志。
CD83 is a surface marker that differentiates immature and mature human dendritic cell populations. Thymic epithelial cell expression of CD83 is also necessary for efficient CD4(+) T cell development in mice. The altered phenotypes of peripheral B and CD4(+) T cells, and the reduction of peripheral CD4(+) T cells in CD83(-/-) mice, suggest additional functions for CD83. To assess this, a panel of mAbs was generated to characterize mouse CD83 expression by peripheral leukocytes. As in humans, activation of conventional and plasmacytoid murine dendritic cell subsets led to rapid up-regulation of CD83 surface expression in mice. In primary and secondary lymphoid compartments, a subset of B cells expressed low-level CD83, while CD83 was not detected on resting T cells. However, CD83 was prominently up-regulated on the majority of spleen B and T cells within hours of activation in vitro. In Vivo, a low dose of hen egg lysozyme (1 mu g) induced significant CD83 but not CD69 expression by Ag-specific B cells within 4 h of Ag challenge. Although B cell development appeared normal in CD83(-/-) mice, B and CD4(+) T cell expression of CD83 was required for lymphocyte longevity in adoptive transfer experiments. Thus, the restricted expression pattern of CD83, its rapid induction following B cell and T cell activation, and its requirement for B cell and CD4(+) T cell longevity demonstrate that CD83 is a functionally significant and sensitive marker of early lymphocyte activation in vivo.