Biophysics of Membrane Stiffening by Cholesterol and Phosphatidylinositol 4,5-bisphosphate (PIP2)
Biophysics of Membrane Stiffening by Cholesterol and Phosphatidylinositol 4,5-bisphosphate (PIP2)
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DOI：
10.1007/978-3-031-21547-6_2
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发表时间：
2023-01-01
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影响因子：
--
通讯作者：
Brown, Michael F.
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文献类型：
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作者：
Doole, Fathima T.;Gupta, Sudipta;Brown, Michael F.
细胞膜调节涉及关键细胞功能的广泛现象。胆固醇是真核细胞膜的关键组分，负责细胞组织、膜弹性和其他关键理化参数。除胆固醇外，其他脂质成分如磷脂酰肌醇4，5-二磷酸（PIP 2）在细胞膜中的浓度较低，但也可以在各种细胞功能中发挥重要的调节作用。在这一章中，我们描述了如何固态氘核磁共振（H-2 NMR）光谱与中子自旋回波（NSE）光谱可以通知协同变化，由于胆固醇和PIP 2的脂质分子包装，调制的抗弯刚度的脂质膜。分子自组装的基本结构-性质关系的照明和指向的长度和时间尺度依赖的细胞膜力学，生物活性和膜脂-蛋白质相互作用的显着影响。
Cell membranes regulate a wide range of phenomena that are implicated in key cellular functions. Cholesterol, a critical component of eukaryotic cell membranes, is responsible for cellular organization, membrane elasticity, and other critical physicochemical parameters. Besides cholesterol, other lipid components such as phosphatidylinositol 4,5-bisphosphate (PIP2) are found in minor concentrations in cell membranes yet can also play a major regulatory role in various cell functions. In this chapter, we describe how solid-state deuterium nuclear magnetic resonance (H-2 NMR) spectroscopy together with neutron spin-echo (NSE) spectroscopy can inform synergetic changes to lipid molecular packing due to cholesterol and PIP2 that modulate the bending rigidity of lipid membranes. Fundamental structure-property relations of molecular self-assembly are illuminated and point toward a length and time-scale dependence of cell membrane mechanics, with significant implications for biological activity and membrane lipid-protein interactions.