Glucose metabolism disorder is a risk factor in ethanol exposure induced malformation in embryonic brain.
Glucose metabolism disorder is a risk factor in ethanol exposure induced malformation in embryonic brain.
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DOI：
10.1016/j.fct.2013.07.059
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
Rui-Rong Tan;Yi-Fang Li;Xiao-tan Zhang;Yi-Hua Huang;Yan-ping Wu;Shu-Hua Ouyang;Bun Tsoi;Ruo-Nan Yi;Xuesong Yang;H. Kurihara;Rong-Rong He-Rong
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文献类型：
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作者：
Rui-Rong Tan;Yi-Fang Li;Xiao-tan Zhang;Yi-Hua Huang;Yan-ping Wu;Shu-Hua Ouyang;Bun Tsoi;Ruo-Nan Yi;Xuesong Yang;H. Kurihara;Rong-Rong He-Rong
据报道，产前接触乙醇会导致大脑发育缺陷。在大脑发育过程中，充足的能量来源被认为是必不可少的，葡萄糖被认为是神经元的主要能量来源。本研究探讨了乙醇对胚胎发育畸形及脑葡萄糖代谢的影响。用不同剂量乙醇（0、10、20、40 mg/蛋）处理孵化36 h的鸡胚。发现胚胎脑重量显著降低。此外，我们观察到在中枢神经系统（CNS）的神经丝表达的免疫染色测定明显减少。上述结果表明，乙醇暴露可引起明显的中枢神经系统损害，并导致发育中的脑畸形。机制研究表明，脑组织葡萄糖和乳酸含量、己糖激酶、丙酮酸激酶和乳酸脱氢酶活性呈剂量依赖性降低。同时，葡萄糖转运蛋白1，葡萄糖转运蛋白3和胰岛素样生长因子I在脑中的mRNA水平表现出显着降低基因表达后，乙醇暴露。提示糖代谢紊乱是乙醇暴露致胚胎脑畸形的重要危险因素。
Prenatal exposure to ethanol has been reported to cause developmental defects in the brain. During brain development, a sufficient energy source is deemed essential and glucose is regarded as the primary energy source for neurons. In this study, the impact of ethanol on embryonic malformation and cerebral glucose metabolism in developing embryo was investigated. Different doses of ethanol (0, 10, 20, 40 mg/egg) were administrated to chicken embryos after 36 h incubation. Embryonic brain weight was found significantly decreased. Moreover, we observed an obvious reduction of neurofilament expression in the central nervous system (CNS) by immunostaining assay. All the above indicated that ethanol exposure caused obvious CNS damages and resulted malformations in the developing brain. Mechanism research showed that cerebral glucose and lactic acid contents, activities of hexokinase, pyruvate kinase and lactic dehydrogenase were decreased dose dependently. Meanwhile, mRNA levels of glucose transporter 1, glucose transporter 3 and insulin-like growth factor I in the brain demonstrated a significant decrease in gene expression after ethanol exposure. These results suggested that glucose metabolism disorder is an important risk factor in ethanol exposure induced malformation in embryonic brain.