Automated measurement of the static light scattering of macromolecular solutions over a broad range of concentrations
Automated measurement of the static light scattering of macromolecular solutions over a broad range of concentrations
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DOI：
10.1016/j.ab.2008.06.032
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发表时间：
2008-10-15
影响因子：
2.9
通讯作者：
Minton, Allen P.
中科院分区：
文献类型：
--
作者：
Fernandez, Cristina;Minton, Allen P.
描述了用于自动测量蛋白质溶液在宽浓度范围内的静态光散射的浓度依赖性的方法和装置。蛋白质浓度梯度是通过连续稀释散射测量池中包含的初始浓缩溶液而产生的，该溶液保持恒定的总体积。通过测量浓度高达 130 g/L 的牛血清白蛋白、卵清蛋白和卵类粘蛋白的光散射浓度依赖性来验证该方法。从所有三种蛋白质获得的实验获得的散射的浓度依赖性在数量上与在测量的浓度范围内没有发生显着的自缔合的假设一致。由爱思唯尔公司出版
A method and apparatus for automated measurement of the concentration dependence of static light scattering Of Protein solutions over a broad range of concentrations is described. The gradient of protein concentrations is created by successive dilutions of an initially concentrated solution contained within the scattering measurement cell, which is maintained at constant total volume. The method is validated by measurement of the concentration dependence of light scattering of bovine serum albumin, ovalbumin, and ovomucoid at concentrations up to 130 g/L. The experimentally obtained concentration dependence of scattering obtained from all three proteins is quantitatively consistent with the assumption that no significant self-association occurs over the measured range of concentrations. Published by Elsevier Inc.