Free radical-induced tandem base damage in DNA oligomers
Free radical-induced tandem base damage in DNA oligomers
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DOI：
10.1016/s0891-5849(97)00166-4
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发表时间：
1997-01-01
影响因子：
7.4
通讯作者：
Freund, HG
中科院分区：
文献类型：
--
作者：
Box, HC;Budzinski, EE;Freund, HG
在脱氧水溶液中X射线照射的两种DNA寡聚体d（GpT）和d（CpGpTpA）中发现了一种新的串联碱基损伤。在这种损伤中，胸腺嘧啶的C6碳原子被羟基化，并且在胸腺嘧啶的C5碳原子和相邻鸟嘌呤碱基的C8碳原子之间形成共价键。此外，质谱数据的形式提出了进一步的证据，证实了以前报道的串联基地病变的结构，电离辐射在氧气的存在下产生的。提出了新的数据上的患病率，以前报道的串联基地病变，其中胸腺嘧啶的甲基碳原子共价连接到相邻的鸟嘌呤基地的C8碳原子。自由基引发的过程中产生的串联基损伤进行了讨论。到目前为止，已经确定了四种不同的辐射诱导的串联基底病变。有证据表明，串联碱基损伤是自由基诱导的DNA损伤的重要组成部分。(C)1997年爱思唯尔科学公司
A new tandem base lesion has been identified in two DNA oligomers, namely d(GpT) and d(CpGpTpA), exposed to X-irradiation in deoxygenated aqueous solution. In this lesion the C6 carbon atom of thymine is hydroxylated and a covalent link is formed between the C5 carbon atom of thymine and the C8 carbon atom of the adjacent guanine base. In addition, further evidence in the form of mass spectrometric data is presented confirming the structures of previously reported tandem base lesions that are produced by ionizing radiation in the presence of oxygen. New data is presented on the prevalence of a previously reported tandem base lesion in which the methyl carbon atom of thymine is covalently linked to the C8 carbon atom of the adjacent guanine base. The free radical-initiated processes by which tandem base damages are generated are discussed. To date four different radiation-induced tandem base lesion have been identified. The evidence suggests that tandem base damage is a significant component of free radical-induced DNA damage. (C) 1997 Elsevier Science Inc.