Characterization of paralogous uncx transcription factor encoding genes in zebrafish
Characterization of paralogous uncx transcription factor encoding genes in zebrafish
复制标题
斑马鱼旁系同源 uncx 转录因子编码基因的表征
DOI：
10.1016/j.gene.2019.100011
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Nittoli V
中科院分区：
文献类型：
--
作者：
Nittoli V
配对型同源结构域转录因子Uncx参与脊椎动物胚胎发生的多个过程。鉴于斑马鱼基因uncx4.1和uncx4.1是小鼠基因uncx的同源基因，我们研究了它们的基因组环境和发育表达。进化分析表明斑马鱼基因是源自硬骨鱼特异性全基因组复制的类似物。斑马鱼胚胎中非转录本的全山situmRNA杂交揭示了新的表达域，证实了先前已知的表达域，并提示了类似物的亚功能化。利用基因突变体和药物抑制剂，我们研究了信号通路在斑马鱼基因表达中的作用。在确定斑马鱼基因的假定功能作用时，我们假设它们编码协调体肌肌生长和神经支配的转录因子。
The paired-type homeodomain transcription factor Uncx is involved in multiple processes of embryogenesis in vertebrates. Reasoning that zebrafish genesuncx4.1anduncxare orthologs of mouseUncx, we studied their genomic environment and developmental expression. Evolutionary analyses indicate the zebrafishuncxgenes as being paralogs deriving from teleost-specific whole-genome duplication. Whole-mountin situmRNA hybridization ofuncxtranscripts in zebrafish embryos reveals novel expression domains, confirms those previously known, and suggests sub-functionalization of paralogs. Using genetic mutants and pharmacological inhibitors, we investigate the role of signaling pathways on the expression of zebrafishuncxgenes in developing somites. In identifying putative functional role(s) of zebrafishuncxgenes, we hypothesized that they encode transcription factors that coordinate growth and innervation of somitic muscles.