Possible Factors Responsible for the Fish-Killing Mechanisms of the Red Tide Phytoplankton, Chattonella marina and Cochlodinium polykrikoides
Possible Factors Responsible for the Fish-Killing Mechanisms of the Red Tide Phytoplankton, Chattonella marina and Cochlodinium polykrikoides
复制标题
赤潮浮游植物、滨海查托氏菌和多核藻的杀鱼机制的可能因素
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
T. Oda
中科院分区：
文献类型：
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作者：
Daekyung Kim;T. Oda
利用ESR自旋捕集技术证实了海洋查顿氏菌产生超氧阴离子（O2 -）和羟基自由基（·OH）等活性氧自由基。几条线索表明，细胞表面结构的C。marina，glycocalyx，具有NADPH依赖的O2产生系统。暴露于C. Marina用抗粗多糖包被的抗血清证明鳃片表面有被抗血清标记的抗原。结果表明，排泌的糖萼在鳃表面的持续积累发生在C。暴露于海水中可能导致ROS介导的严重鳃组织损伤，从而导致鱼类死亡。与C.在海水中，多棱钴甲藻体内O2 -和H2 O2的含量均为痕量水平。此外，C. polykrikoides在凝集素或从皮肤和鳃制备的鱼粘液的存在下观察到，而C.玛丽娜产生的水平增加的O2 -响应这些刺激。采用C. Polykrikoides对HeLa细胞有毒性作用，而C.玛丽娜几乎没有任何效果。此外，在培养过程中，培养基中的多糖逐渐积累。polykrikoides的培养液逐渐变粘稠，而C.码头这些结果表明，C。marina和C. polykrikoides是不同的。ROS可能在C. marina，而在C.对于多糖，某些有毒物质或多糖是毒性的主要原因，而不是ROS。
Generation of reactive oxygen species (ROS) such as superoxide (O2 –) and hydroxyl radical (·OH) by Chattonella marina could be confirmed by ESR spin trapping method. Several lines of evidence suggested that cell surface structure of C. marina, glycocalyx, has NADPH-dependent O2 – generation system. Immunohistochemical analysis of gill lamellae from yellowtail exposed to C. marina using antiserum against crude glycocalyx demonstrated that there were the antigns recognaised by the antiserum on the surface of gill lamellae. The results suggest that continuous accumulation of discharged glycocalyx on the gill surface occurs during C. marina exposure, which may be responsible for the ROSmediated severe gill tissue damage leading to fish death. As compare to C. marina, the levels of O2 – and H2O2 detected in Cochlodinium polykrikoides were trace levels. Furthermore, no significant increase in O2 – generation by C. polykrikoides was observed in the presence of lectins or fish mucus prepared from skin and gill of yellowtail, whereas C. marina generated increased level of O2 – responding to these stimuli. The cell-free aqueous extract prepared from C. polykrikoides showed toxic effect on HeLa cells, but the extract of C. marina had almost no effect. Furthermore, gradual accumulation of polysaccharides in the medium was observed during the culture of C. polykrikoides, and the medium gradually became viscous, but no such change was observed in the medium of C. marina. These results suggest that ichthyotoxic mechanisms of C. marina and C. polykrikoides are different. ROS may play a significant role in the fish-killing activity of C. marina, whereas in the case of C. polykrikoides, certain toxic substances or polysaccharides are mainly responsible for the toxicity rather than ROS.
Cerulenin 抑制布雷菲德菌素 A 抗性细胞系中蓖麻毒素、莫德菌素、假单胞菌毒素和白喉毒素的细胞毒性。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Oda,T;Wu,HC
通讯作者：
Wu,HC