Plasmid profiles compared with serotyping and pyocin typing for epidemiological surveillance of Pseudomonas aeruginosa.
Plasmid profiles compared with serotyping and pyocin typing for epidemiological surveillance of Pseudomonas aeruginosa.
复制标题
质粒谱与血清分型和化脓菌素分型比较，用于铜绿假单胞菌的流行病学监测。
DOI：
10.1099/00222615-25-2-109
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发表时间：
1988
影响因子：
3
通讯作者：
T. Bergan
中科院分区：
文献类型：
--
作者：
Chit Laa Poh;E. Yap;L. Tay;T. Bergan
采用两种快速、重复性好的质粒筛选方法对112株铜绿假单胞菌进行质粒谱测定。质粒DNA存在于15%的菌株检查。原生质体大小在1.2 × 10 ~（6）~ 60.2 × 10 ~（6）mol.重量优势血清型为O：11和O：4，分别占菌株的38%和12%。四种绿脓菌素类型（1、10、3和5）占优势（各自的频率：56、15、12和6%）。绿脓菌素分型的再现性明显劣于质粒谱分析和血清分型。相同血清型的菌株可以通过不同的质粒谱进一步区分。血清学上无法分型或多聚凝集菌株成功地通过质粒图谱模式进行了表征。因此，质粒谱分析显示是铜绿假单胞菌流行病学分型血清分型的有用辅助手段。
The plasmid profiles of 112 clinical isolates of Pseudomonas aeruginosa were determined by two reproducible, rapid, plasmid screening methods. Plasmid DNA was present in 15% of isolates examined. Plasmids varied in size from 1.2 X 10(6) to 60.2 X 10(6) mol. wt. The dominant serotypes encountered were O:11 and O:4, which comprised 38% and 12% of strains, respectively. Four pyocin types (1, 10, 3 and 5) dominated (respective frequencies: 56, 15, 12 and 6%). Reproducibility of pyocin typing was distinctly inferior to both plasmid profiling and serotyping. Strains of identical serotypes could be further differentiated by dissimilar plasmid profiles. Serologically untypable or polyagglutinable strains were successfully characterised by plasmid profile patterns. Thus, plasmid profiling was shown to be a useful adjunct to serotyping for the epidemiological typing of P. aeruginosa.