The molecular pathophysiology of subacromial bursitis in rotator cuff disease
The molecular pathophysiology of subacromial bursitis in rotator cuff disease
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DOI：
10.1016/j.jse.2004.09.022
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发表时间：
2005-01-01
影响因子：
3
通讯作者：
Bigliani, LU
中科院分区：
文献类型：
--
作者：
Blaine, TA;Kim, YS;Bigliani, LU
关于肩峰滑囊炎和肩袖疾病的分子和生化病理生理学的信息很少。我们研究了肩袖疾病患者肩峰下囊中细胞因子（白细胞介素 [IL]-1β、IL-1、IL-6、肿瘤坏死因子 [TNF] α、小诱导型细胞因子）、金属蛋白酶和环氧合酶的表达模式。按照机构审查委员会批准的方案，准备肩峰下囊标本，用于接受肩部手术的患者的分子和生化分析。通过使用基因表达微阵列和免疫组织细胞化学分析样本中细胞因子、金属蛋白酶和环氧合酶的存在。基因表达和免疫组织化学的微阵列分析表明，与对照标本相比，肩峰滑囊炎患者的多种细胞因子基因（TNF、IL-1α、IL-1β和IL-6）的表达增加，此外，滑囊炎组的金属蛋白酶（MMP-1和MMP-9）和环加氧酶（COX-1和COX-2）的表达也比对照标本增加。尽管还需要进一步研究，但这些研究表明肩袖疾病患者确实会发生肩峰下囊炎症。这些发现支持抗炎药在治疗肩峰下撞击症中的作用，并强调肩峰下滑囊切除术对于减少肩袖疾病炎症的重要性。
Little information exists on the molecular and biochemical pathophysiology of subacromial bursitis and rotator cuff disease. We investigated the pattern of expression of cytokines (interleukin [IL]- 1beta, IL- 1, IL-6, tumor necrosis factor [TNF] alpha, small inducible cytokines), metalloproteases, and cyclooxygenases in the subacromial bursa in patients with rotator cuff disease. Subacromial bursa specimens were prepared for molecular and biochemical analysis in patients undergoing shoulder surgery following an institutional review board-approved protocol. Specimens were analyzed for the presence of cytokines, metalloproteases, and cyclooxygenases by use of microarray for gene expression and immunohistocytochemistry. Microarray analysis for gene expression and immunohistochemistry demonstrated that the expression of several cytokine genes (TNF, IL-1alpha, IL-1beta, and IL-6) was increased in patients with subacromial bursitis compared with control specimens, Furthermore, the expression of metalloproteases (MMP-1 and MMP-9) and cyclooxygenases (COX-1 and COX-2) in the bursitis group was found to be increased as compared with controls. Although further investigation is required, these studies suggest that inflammation of the subacromial bursa does occur in patients with rotator cuff disease. These findings support the role of anti-inflammatory agents in the treatment of subacromial impingement and emphasize the importance of subacromial bursectomy to reduce inflammation in rotator cuff disease.