ORFeus: detection of distant homology using sequence profiles and predicted secondary structure
ORFeus: detection of distant homology using sequence profiles and predicted secondary structure
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DOI：
10.1093/nar/gkg504
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发表时间：
2003-07-01
影响因子：
14.9
通讯作者：
Rychlewski, L
中科院分区：
文献类型：
--
作者：
Ginalski, K;Pas, J;Rychlewski, L
Orfeus是通过结构预测元服务器（http://bioinfo.pl/meta/）来提供学术社区的全自动，敏感蛋白质序列相似性搜索服务器。 Orfeus发展的目的是提高远距离相关蛋白质家族的检测的敏感性。预测的二级结构信息被添加到有关序列保守和可变性的信息中，这是一种从混合螺纹方法中知道的技术。将以这种方式创建的元配置文件的精度与仅包含序列信息的轮廓以及将单个序列与配置文件对齐的标准方法进行了比较。此外，比对齐两个仅序列谱图或将元素对齐序列与序列对齐，对蛋白质家族之间的远程同源性的比对更敏感。与仅稳健序列的曲线相比，在较低特异性范围内，对齐得分的特异性得到了提高。
ORFeus is a fully automated, sensitive protein sequence similarity search server available to the academic community via the Structure Prediction Meta Server (http://BioInfo.PL/Meta/). The goal of the development of ORFeus was to increase the sensitivity of the detection of distantly related protein families. Predicted secondary structure information was added to the information about sequence conservation and variability, a technique known from hybrid threading approaches. The accuracy of the meta profiles created this way is compared with profiles containing only sequence information and with the standard approach of aligning a single sequence with a profile. Additionally, the alignment of meta profiles is more sensitive in detecting remote homology between protein families than if aligning two sequence-only profiles or if aligning a profile with a sequence. The specificity of the alignment score is improved in the lower specificity range compared with the robust sequence-only profiles.