Building programmable jigsaw puzzles with RNA
Building programmable jigsaw puzzles with RNA
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DOI：
10.1126/science.1104686
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发表时间：
2004-12-17
期刊：
影响因子：
56.9
通讯作者：
Jaeger, L
中科院分区：
文献类型：
--
作者：
Chworos, A;Severcan, I;Jaeger, L
超分子化学的一个挑战是设计多功能的自组装结构单元，以实现在分子水平上对物质排列的完全控制。我们已经实现了对人工RNA构建块的三维结构的可靠预测和设计，以生成称为tectosquares的分子拼图单元。它们可以通过控制其几何形状、拓扑结构、方向性和可寻址性来编程，以算法自组装成具有预定义的周期性和非周期性图案以及有限尺寸的各种复杂的纳米级织物。这项工作强调了RNA的模块化和层次化特征，表明小RNA结构基序可以编码大分子结构的精确拓扑结构。它证明了基于RNA的完全可寻址材料可以合成，并提供了对涉及大量RNA分子的自组装过程的见解。
One challenge in supramolecular chemistry is the design of versatile, self-assembling building blocks to attain total control of arrangement of matter at a molecular level. We have achieved reliable prediction and design of the three-dimensional structure of artificial RNA building blocks to generate molecular jigsaw puzzle units called tectosquares. They can be programmed with control over their geometry, topology, directionatity, and addressability to algorithmically self-assemble into a variety of complex nanoscopic fabrics with predefined periodic and aperiodic patterns and finite dimensions. This work emphasizes the modular and hierarchical characteristics of RNA by showing that small RNA structural motifs can code the precise topology of large molecular architectures. It demonstrates that fully addressable materials based on RNA can be synthesized and provides insights into self-assembly processes involving large populations of RNA molecules.