Amplified Fluorescent Recognition of G-Quadruplex Folding with a Cationic Conjugated Polymer and DNA Intercalator
Amplified Fluorescent Recognition of G-Quadruplex Folding with a Cationic Conjugated Polymer and DNA Intercalator
复制标题
使用阳离子共轭聚合物和 DNA 嵌入剂增强 G-四链体折叠的荧光识别
DOI：
10.1021/am1006854
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发表时间：
2010-11-01
影响因子：
9.5
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Xu, Hui;Gao, Shuli;Fan, Chunhai
富含G的单链DNA在配体的存在下，通过分子内氢键作用可折叠成G-四链体。这种结构转换可以通过基于SYBR绿色I（SG）与各种DNA结构之间的不同相互作用的荧光方法特异性地检测。结果表明，SG可以嵌入G-四链体中，并产生高荧光强度，由于带正电的阳离子共轭聚合物（CCP）与带负电的刚性G-四链体的高亲和力，通过荧光共振能量转移（FRET），从CCP到SG的荧光强度可以进一步放大6倍，而在无K存在的情况下，ssDNA的荧光强度不能被放大。因此，ssDNA/SG/CCP复合物可以用于以无标记和成本有效的方式检测具有改进的选择性的钾离子。
The single stranded DNA (ssDNA) with G-rich sequence can fold into G-quadruplex vial intramolecular hydrogen bonding interaction in the presence of ligand. This structure conversion can be specifically detected by a fluorescence method based on different interaction between SYBR Green I (SG) and various DNA structures. SG is proved to intercalate into G-quadruplex and results in high fluorescence intensity which can be further amplified by 6-fold through fluorescence resonance energy transfer (FRET) from a water-soluble cationic conjugated polymer (CCP) to SG due to the high affinity of positively charged CCP to negatively charged rigid G-quadruplex whereas it is not performed for ssDNA in the absence of K. As a result, the ssDNA/SG/CCP complex can be used to detect potassium ions with improved selectivity in a label-free and cost-effective manner.