Effect of the spin-trapping compound N-tert-butyl-alpha-phenylnitrone on protein oxidation and life span.
Effect of the spin-trapping compound N-tert-butyl-alpha-phenylnitrone on protein oxidation and life span.
复制标题
自旋捕获化合物 N-叔丁基-α-苯基硝酮对蛋白质氧化和寿命的影响。
DOI：
10.1006/abbi.1995.0037
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Sohal,RS
中科院分区：
文献类型：
--
作者：
Dubey,A;Forster,MJ;Sohal,RS
n-叔丁基-α-苯基硝基酮（PBN）是一种亲脂性自旋捕获化合物，据报道，它可以将老年沙鼠大脑中蛋白质氧化产物——蛋白质羰基的浓度降低到几乎与幼年沙鼠相同的水平(Carney， J. M.,et al.(1991)Proc。国家的。学会科学。USA88, 3633 - 3636)。最近，Cao和Cutler (1995)Arch对这一发现的有效性以及通常用于测量蛋白质羰基的2,4-二硝基苯肼方法提出了质疑。物化学。Biophys.320, 106 - 114)。本研究的目的是探讨一些相关的问题，例如：(a)关于PBN作用的最初发现是否可以得到证实，(b) PBN是否在其他物种和组织中发生类似的作用，以及(c) PBN是否影响动物的寿命。本研究的结果证实了最初的发现，即PBN确实导致沙鼠大脑皮层中蛋白质羰基含量的降低。然而，在沙鼠的心脏或老鼠的大脑皮层中没有观察到类似的效果。因此，PBN对蛋白质羰基的影响因组织和物种而异。PBN没有延长家蝇的寿命，并且在相对高浓度时被发现是有毒的。
N-tert-Butyl-α-phenylnitrone (PBN), a lipophilic spin-trapping compound, has been reported to decrease the concentration of protein carbonyls, the products of protein oxidation, in the brain of old gerbils to virtually the level found in the young gerbils (Carney, J. M.,et al.(1991)Proc. Natl. Acad. Sci. USA88, 3633–3636). The validity of this finding as well as that of the commonly used 2,4-dinitrophenylhydrazine procedure for the measurement of protein carbonyls was recently called into question by Cao and Cutler ((1995)Arch. Biochem. Biophys.320, 106–114). The objective of the present study was to examine some of the relevant issues such as (a) whether the original findings on the effects of PBN can be confirmed, (b) whether similar effects of PBN occur in other species and tissues, and (c) whether PBN affects the life span of animals. Results of this study provide confirmation of the original finding that PBN indeed causes a decrease in protein carbonyl content in the gerbil brain cortex. However, a similar effect is not observed in the gerbil heart or the mouse brain cortex. Effects of PBN on protein carbonyls are thus variable depending upon tissue and species. PBN administration did not extend the life span of houseflies and at relatively high concentrations it was found to be toxic.