Predictor parameters of liver viability during porcine normothermic ex situ liver perfusion in a model of liver transplantation with marginal grafts
Predictor parameters of liver viability during porcine normothermic ex situ liver perfusion in a model of liver transplantation with marginal grafts
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DOI：
10.1111/ajt.15395
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发表时间：
2019-11-01
影响因子：
8.8
通讯作者：
Selzner, Markus
中科院分区：
文献类型：
--
作者：
Linares-Cervantes, Ivan;Echeverri, Juan;Selzner, Markus
常温异位肝灌注（NEsLP）提供了评估移植物功能和损伤的生物标志物的机会。我们研究了NEsLP参数（生物标志物和标记物），用于评估猪移植模型中的肝活力。研究了来自心脏跳动供体（HBD）和来自热缺血30分钟（心源性死亡[DCD]30 '后捐献）、70分钟（DCD 70'）和120分钟（DCD 120 '）供体的移植物。HBD、DCD 30 '和DCD 70'-组的存活率为100%。相比之下，在DCD 120 '-组中，70%的患者发生原发性无功能（PNF）并死亡。NEsLP期间的肝细胞功能显示低乳酸（
Normothermic ex situ liver perfusion (NEsLP) offers the opportunity to assess biomarkers of graft function and injury. We investigated NEsLP parameters (biomarkers and markers) for the assessment of liver viability in a porcine transplantation model. Grafts from heart-beating donors (HBD), and from donors with 30 minutes (donation after cardiac death [DCD]30 '), 70 minutes (DCD70 '), and 120 minutes (DCD120 ') of warm ischemia were studied. The HBD, DCD30 ', and DCD70 '-groups had 100% survival. In contrast, 70% developed primary nonfunction (PNF) and died in the DCD120 '-group. Hepatocellular function during NEsLP showed low lactate (