Biofilm production by Aureobasidium pullulans improves biocontrol against sour rot in citrus
Biofilm production by Aureobasidium pullulans improves biocontrol against sour rot in citrus
复制标题
DOI：
10.1016/j.fm.2017.07.008
复制
发表时间：
2018-02-01
影响因子：
5.3
通讯作者：
Kupper, Katia Cristina
中科院分区：
文献类型：
--
作者：
Klein, Mariana Nadjara;Kupper, Katia Cristina
研究了出芽短梗霉ACBL-77菌株对柑橘酸腐病病原菌地霉的生防效果及其相互作用。为此，利用扫描电子显微镜对添加营养物质优化酵母菌的拮抗活性、微生物之间对营养物质的竞争、营养物质对酵母细胞和生物膜产生的影响以及它们在生防病害、柑橘果实中酵母的存活和微生物之间的相互作用等方面进行了评价。微量营养素(硼酸、氯化钴和钼酸铵)有利于出芽短梗霉的拮抗作用。1%的硫酸铵和0.5%的蔗糖有利于酵母菌之间的竞争。在酵母培养物中添加1%的硫酸铵，促进了生物膜的产生，提高了对该病的拮抗活性，也使酵母菌在柑橘果实伤害处有了更好的存活。这种酵母菌能够在柑橘上形成生物膜，使病原菌菌丝变形。这些结果表明了在以普鲁兰弧菌为基础的配方中添加营养物质的重要性，以期将其用于商业规模。这是首次报道出芽短梗霉生物被膜数量的增加与拮抗活性的增加之间的正相关关系。(C)2017爱思唯尔有限公司。保留所有权利。
The biocontrol efficiency of Aureobasidium pullulans strain ACBL-77 against Geotrichum citri-aurantii, causal agent of sour rot in citrus, and their interactions were evaluated. For this, were evaluated the incorporation of nutrients in optimizing the antagonistic activity of the yeast, the competition for nutrients between microorganisms, the effect of nutrients on yeast cell and biofilm production and their correlation in the biocontrol of disease, the survival of yeast in citrus fruits and the interaction between microorganisms using scanning electron microscopy. Micronutrients (boric acid, cobalt chloride and ammonium molybdate) favoured the antagonistic action of A. pullulans. Ammonium sulfate 1% and sucrose 0.5% favoured the yeast during the competition between the microorganisms. The addition of ammonium sulfate (1%) in the yeast culture stimulated biofilm production and increased the antagonistic activity against the disease, as also allowed the better survival of yeast in wounded sites of citrus fruit. The yeast was found to be able to form biofilms on citrus, deforming the pathogen hyphae. These results showed the importance of the addition of nutrients in A. pullulans based-formulations when aiming for their use on a commercial scale. This is the first report of a positive correlation between the increase in the quantity of biofilm produced by A. pullulans, with increased antagonistic activity. (C) 2017 Elsevier Ltd. All rights reserved.