Rapid and multiplex microRNA detection on graphically encoded silica suspension array
Rapid and multiplex microRNA detection on graphically encoded silica suspension array
复制标题
对图形编码的二氧化硅悬浮阵列进行快速、多重 microRNA 检测
DOI：
10.1016/j.bios.2014.05.020
复制
发表时间：
2014
影响因子：
12.6
通讯作者：
Li Jiong
中科院分区：
文献类型：
--
作者：
Jiang Li;Shen Ye;Zheng Kexiao;Li Jiong
MicroRNA(MiRNA)是一种18-24个核苷酸的非编码RNA分子，已成为临床诊断癌症的一类理想的候选生物标志物。到目前为止，已经在平面阵列和悬浮阵列上发展了许多miRNAs的检测方法。在这项工作中，我们描述了一种杂交触发的荧光策略，用于在图形编码的二氧化硅悬浮阵列上进行无标记和多重miRNA检测。总RNA直接应用于8聚体通用标签的分析，该标签可以通过碱基堆积效应被捕获探针选择性地捕获。得益于碱基堆积效应，这种新方法对5‘端和3’端的单核苷酸改变表现出极好的识别能力。成熟的miRNAs很容易与其对应的前miRNAs区分开来。此外，估计的检测下限为5摩尔，可与一些最灵敏的方法相媲美。所有这些特征都为发现和临床应用提供了令人兴奋的可能性。
MicroRNA (miRNA), an 18–24-nucleotide noncoding RNA molecule, has become an ideal class of biomarker candidates for clinical diagnosis of cancers. By now, a number of detection methods for miRNAs have been developed on planar arrays and suspension arrays. In this work, we describe a hybridization-triggered fluorescence strategy for label-free and multiplex miRNA detection on graphically encoded silica suspension array. The total RNA is directly applied for analysis with an 8-mer Universal Tag which can be selectively captured by the capture probe via base-stacking effects. Benefiting from base-stacking effects, this novel method exhibits superb discrimination ability toward the 5′ and 3′ end single-nucleotide alteration. Mature miRNAs can be distinguished from their corresponding pre-miRNAs easily. Moreover, the estimated detection limit of 5 amol is comparable to some of the most sensitive methods. All these mentioned characteristics offer exciting possibilities for discovery and clinical applications.