Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes
Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes
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DOI：
10.1099/vir.0.82456-0
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发表时间：
2007-02-01
影响因子：
3.8
通讯作者：
Mertens, P. P. C.
中科院分区：
文献类型：
--
作者：
Maan, S.;Maan, N. S.;Mertens, P. P. C.
蓝舌病病毒（BTV）的外壳蛋白VP 2是哺乳动物宿主产生的保护性免疫应答的靶标。VP 2含有大多数被中和抗体识别的表位，因此也是BTV血清型的主要决定因素。合成、克隆和测序了来自24种BTV血清型中的每一种的参考株的基因组片段2（Seg-2，其编码VP 2）的全长cDNA拷贝。这代表了第一个完整的全长BTV VP 2基因集（来自24种血清型）已被分析。每个Seg-2具有单个开放阅读框，具有与保守的末端六核苷酸序列相邻的短反向重复序列。这些数据表明Seg-2中的总体血清型间变异为29%（BTV-8和BTV-18）至59%（BTV-16和13 TV-22），而VP 2的推导氨基酸序列为22.4%（BTV-4和BTV-20）至73%（13 TV-6和BTV-22）。检测到10种不同的Seg-2谱系（核型），这些血清型之间的序列相似性最大，以前曾报告为血清学“相关”。在已报道为抗原性重要的VP 2区域中，在不同血清型之间观察到较少的相似性，表明它们可能在中和抗体应答中发挥作用。所提供的数据形成了通过序列分析和Seg-2比较进行BTV血清型鉴定的初步基础，并用于开发针对单个BTV血清型的分子诊断检测（通过RT-PCR）。
The outer capsid protein VP2 of Bluetongue virus (BTV) is a target for the protective immune response generated by the mammalian host. VP2 contains the majority of epitopes that are recognized by neutralizing antibodies and is therefore also the primary determinant of BTV serotype. Full-length cDNA copies of genome segment 2 (Seg-2, which encodes VP2) from the reference strains of each of the 24 BTV serotypes were synthesized, cloned and sequenced. This represents the first complete set of full-length BTV VP2 genes (from the 24 serotypes) that has been analysed. Each Seg-2 has a single open reading frame, with short inverted repeats adjacent to conserved terminal hexanucleotide sequences. These data demonstrated overall inter-serotype variations in Seg-2 of 29 % (BTV-8 and BTV-18) to 59 % (BTV-16 and 13TV-22), while the deduced amino acid sequence of VP2 varied from 22.4 % (BTV-4 and BTV-20) to 73 % (13TV-6 and BTV-22). Ten distinct Seg-2 lineages (nucleotypes) were detected, with greatest sequence similarities between those serotypes that had previously been reported as serologically 'related'. Fewer similarities were observed between different serotypes in regions of VP2 that have been reported as antigenically important, suggesting that they may play a role in the neutralizing antibody response. The data presented form an initial basis for BTV serotype identification by sequence analyses and comparison of Seg-2, and for development of molecular diagnostic assays for individual BTV serotypes (by RT-PCR).