A comparison of the performance and application differences between manual and automated patch-clamp techniques.
A comparison of the performance and application differences between manual and automated patch-clamp techniques.
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DOI：
10.2174/1875397301206010087
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Zhiyuan L
中科院分区：
文献类型：
--
作者：
Yajuan X;Xin L;Zhiyuan L
膜片钳技术常用于电生理学实验，通过表征离子通道活性，提供对离子通道特性的直接洞察。该技术可用于阐明药物与特定离子通道在不同构象状态下的相互作用，以了解离子通道调节剂的作用机制。膜片钳技术被认为是离子通道研究的金标准，然而，它具有低通量和高人员成本的缺点。在过去的十年中，几个自动化电生理平台的发展大大增加了全细胞电生理记录的屏幕吞吐量。自动膜片钳系统的新进展旨在提供高数据质量、高内容和高通量。然而，由于上述限制，在离子通道研究中，自动膜片钳系统不能取代手动膜片钳系统。虽然自动膜片钳系统可用于在稳定表达高水平离子通道的细胞系中筛选大量化合物，但手动膜片钳技术仍然是研究某些研究领域和特定细胞类型的离子通道特性所必需的，包括具有混合细胞类型的原代细胞和衍生自诱导多能干细胞（iPSC）或胚胎干细胞（ESC）的分化细胞。因此，进一步提高细胞类型和数据质量的灵活性将拓宽自动膜片钳系统在学术界和工业界的应用。
The patch clamp technique is commonly used in electrophysiological experiments and offers direct insight into ion channel properties through the characterization of ion channel activity. This technique can be used to elucidate the interaction between a drug and a specific ion channel at different conformational states to understand the ion channel modulators’ mechanisms. The patch clamp technique is regarded as a gold standard for ion channel research; however, it suffers from low throughput and high personnel costs. In the last decade, the development of several automated electrophysiology platforms has greatly increased the screen throughput of whole cell electrophysiological recordings. New advancements in the automated patch clamp systems have aimed to provide high data quality, high content, and high throughput. However, due to the limitations noted above, automated patch clamp systems are not capable of replacing manual patch clamp systems in ion channel research. While automated patch clamp systems are useful for screening large amounts of compounds in cell lines that stably express high levels of ion channels, the manual patch clamp technique is still necessary for studying ion channel properties in some research areas and for specific cell types, including primary cells that have mixed cell types and differentiated cells that derive from induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs). Therefore, further improvements in flexibility with regard to cell types and data quality will broaden the applications of the automated patch clamp systems in both academia and industry.