Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics.
Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics.
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DOI：
10.1091/mbc.e15-10-0694
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发表时间：
2017-02-01
影响因子：
3.3
通讯作者：
Al-Bassam J
中科院分区：
文献类型：
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作者：
Al-Bassam J
可溶性αβ-微管蛋白异源二聚体在真核细胞内保持高浓度，形成从根本上驱动微管动力学的池。5个保守的微管蛋白辅因子和ADP核糖基化因子样2调节αβ-微管蛋白的生物发生和降解，以维持浓缩的可溶性池。在这里，我描述了一个修改后的模型，三个微管蛋白辅因子和Arl 2作为一个多亚基GTP水解催化伴侣，循环，以促进αβ-微管蛋白的生物合成和降解的功能。该模型有助于解释新旧数据，表明这些活动通过修复或去除可溶性池中的αβ-微管蛋白来增强体内微管动力学
Soluble αβ-tubulin heterodimers are maintained at high concentration inside eukaryotic cells, forming pools that fundamentally drive microtubule dynamics. Five conserved tubulin cofactors and ADP ribosylation factor–like 2 regulate the biogenesis and degradation of αβ-tubulins to maintain concentrated soluble pools. Here I describe a revised model for the function of three tubulin cofactors and Arl2 as a multisubunit GTP-hydrolyzing catalytic chaperone that cycles to promote αβ-tubulin biogenesis and degradation. This model helps explain old and new data indicating these activities enhance microtubule dynamics in vivo via repair or removal of αβ-tubulins from the soluble pools