Expression of the periostin mRNA level in neuroblastoma
Expression of the periostin mRNA level in neuroblastoma
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DOI：
10.1053/jpsu.2002.34985
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发表时间：
2002-09-01
影响因子：
2.4
通讯作者：
Fuji, Y
中科院分区：
文献类型：
--
作者：
Sasaki, H;Sato, Y;Fuji, Y
背景：神经母细胞瘤是儿童早期最常见的恶性实体瘤。神经母细胞瘤的诊断无论是通过大规模筛查还是根据临床症状，都与疾病的预后有关。Periostin蛋白与昆虫黏附分子Fasciclin 1在结构和序列上具有同源性。方法：应用LightCycler实时逆转录聚合酶链式反应(RT-PCR)检测24例神经母细胞瘤组织中Periostin信使RNA的表达。结果：与I期相比，IV期肿瘤标本中Periostin转录水平有升高的趋势(P=.0845)。症状诊断组的Periostin mRNA水平高于大规模筛查组(P=0.0266)。神经母细胞瘤中1岁以后确诊组的Periostin mRNA表达水平也高于1岁前确诊组(P=0.0059)。结论：神经母细胞瘤中Periostin mRNA的表达水平与肿瘤进展或预后有关。版权所有2002，《爱思唯尔科学》(美国)。版权所有。
Background: Neuroblastoma is the most common malignant solid tumor in early childhood. Whether neuroblastoma is diagnosed by mass screening or by clinical symptom, it has been reported to be correlated with prognosis of the disease. The periostin protein shares structural and sequence homology with fasciclin 1, which is an insect adhesion molecule.Methods: Expression of periostin messenger RNAs were evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR) assay in 24 tumor samples from neuroblastoma using LightCycler. The data were analyzed in reference to clinicopathologic factors.Results: There was a tendency for higher periostin transcripts levels in the tumor samples from stage IV when compared with the stage I (P = .0845). The periostin mRNA level was higher in the group with disease diagnosed by symptom than in the group with disease diagnosed by mass screening (P = .0266). The periostin mRNA level also was higher in the group with disease diagnosed after 1 year of age than in the group with disease diagnosed before 1 year of age (P = .0059).Conclusion: Because the groups with disease diagnosed by symptom or after 1 year of age were reported to be the worse prognosis, the periostin mRNA expression levels were correlated with tumor progression or prognosis of neuroblastoma. Copyright 2002, Elsevier Science (USA). All rights reserved.